Freeze-dried and GMP-compliant pharmaceuticals containing exosomes for acellular mesenchymal stromal cell immunomodulant therapy

Elia Bari, Sara Perteghella, Laura Catenacci, Marzio Sorlini, Stefania Croce, Melissa Mantelli, Maria A. Avanzini, Milena Sorrenti, Maria L. Torre

Research output: Contribution to journalArticle

Abstract

Aim: To validate the use of ultrafiltration (UF) as an alternative applicable industrial method to replace ultracentrifugation (UC) in the purification of mesenchymal stromal cell (MSC)-secretome. Materials & methods: Pharmaceutical formulations containing secretome and/or extracellular vesicles were extracted from adipose-MSCs and bone marrow-MSCs by combining UF or UC with lyophilization. Results & conclusion: UF led to higher protein, lipid, cytokine and exosomes yields compared with UC. The isolation procedure and cell source influenced immunomodulatory activity, which was in vitro evaluated by inhibition of phytohemagglutinin-activated peripheral blood mononuclear cell proliferation, and by modulation of IL-10, IFN-γ and IL-6. A secretome dosage was identified to obtain the same immunomodulatory activity of MSCs, paving the way for cell-free therapy.

Original languageEnglish
Pages (from-to)753-765
Number of pages13
JournalNanomedicine
Volume14
Issue number6
DOIs
Publication statusPublished - Mar 1 2019

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Exosomes
Ultracentrifugation
Ultrafiltration
Cell- and Tissue-Based Therapy
Mesenchymal Stromal Cells
Drug products
Pharmaceutical Preparations
Drug Compounding
Freeze Drying
Cell Separation
Cell proliferation
Phytohemagglutinins
Interleukin-10
Lipids
Purification
Interleukin-6
Blood Cells
Bone
Blood
Bone Marrow

Keywords

  • adipose tissue
  • bone marrow
  • exosome
  • extracellular vesicles
  • freeze-drying
  • immunomodulation
  • mesenchymal stromal cells
  • secretome
  • ultracentrifugation
  • ultrafiltration

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomedical Engineering
  • Materials Science(all)

Cite this

Freeze-dried and GMP-compliant pharmaceuticals containing exosomes for acellular mesenchymal stromal cell immunomodulant therapy. / Bari, Elia; Perteghella, Sara; Catenacci, Laura; Sorlini, Marzio; Croce, Stefania; Mantelli, Melissa; Avanzini, Maria A.; Sorrenti, Milena; Torre, Maria L.

In: Nanomedicine, Vol. 14, No. 6, 01.03.2019, p. 753-765.

Research output: Contribution to journalArticle

Bari, Elia ; Perteghella, Sara ; Catenacci, Laura ; Sorlini, Marzio ; Croce, Stefania ; Mantelli, Melissa ; Avanzini, Maria A. ; Sorrenti, Milena ; Torre, Maria L. / Freeze-dried and GMP-compliant pharmaceuticals containing exosomes for acellular mesenchymal stromal cell immunomodulant therapy. In: Nanomedicine. 2019 ; Vol. 14, No. 6. pp. 753-765.
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AU - Croce, Stefania

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