Frequency and multiplicity of human papillomavirus infection in HIV-1 positive women in Italy

Research output: Contribution to journalArticle

Abstract

Background: Natural history of HPV infection is altered in HIV positive women, with increased risk of cervical dysplasia. Limited data are available about the influence of HPV genotypes and HPV multiple infections on cervical disease in HIV positive women. Objectives: We determined HPV genotype prevalence in cervical samples from 553 HIV-infected women attending the gynaecological service at " L. Spallanzani" Hospital. Association of HPV multiple infections with cervical abnormalities was investigated. Study design: MY09/MY11 consensus primers were used to detect HPV-DNA; HPV typing was performed by RFLP. A commercial array based kit was used to define unresolved RFLP patterns. Results: HPV was detected in 244/553 (44.1%) patients, correlating with low CD4 counts (p3). Dyskaryosis was associated with decreased CD4 counts (≥500 vs 200-499 vs 3, χ 2 for trend, p=0.001) and with HPV types multiplicity (1 vs 2-3 vs ≥4, χ 2 for trend, p

Original languageEnglish
Pages (from-to)141-146
Number of pages6
JournalJournal of Clinical Virology
Volume54
Issue number2
DOIs
Publication statusPublished - Jun 2012

Fingerprint

Papillomavirus Infections
Italy
HIV-1
HIV
CD4 Lymphocyte Count
Restriction Fragment Length Polymorphisms
Infection
Genotype
Uterine Cervical Dysplasia
DNA Fingerprinting
Natural History

Keywords

  • Cervical lesions
  • H-SIL
  • HIV
  • HPV multiple infection
  • HPV prevalence

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

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title = "Frequency and multiplicity of human papillomavirus infection in HIV-1 positive women in Italy",
abstract = "Background: Natural history of HPV infection is altered in HIV positive women, with increased risk of cervical dysplasia. Limited data are available about the influence of HPV genotypes and HPV multiple infections on cervical disease in HIV positive women. Objectives: We determined HPV genotype prevalence in cervical samples from 553 HIV-infected women attending the gynaecological service at {"} L. Spallanzani{"} Hospital. Association of HPV multiple infections with cervical abnormalities was investigated. Study design: MY09/MY11 consensus primers were used to detect HPV-DNA; HPV typing was performed by RFLP. A commercial array based kit was used to define unresolved RFLP patterns. Results: HPV was detected in 244/553 (44.1{\%}) patients, correlating with low CD4 counts (p3). Dyskaryosis was associated with decreased CD4 counts (≥500 vs 200-499 vs 3, χ 2 for trend, p=0.001) and with HPV types multiplicity (1 vs 2-3 vs ≥4, χ 2 for trend, p",
keywords = "Cervical lesions, H-SIL, HIV, HPV multiple infection, HPV prevalence",
author = "Garbuglia, {Anna Rosa} and Pierluca Piselli and Daniele Lapa and Catia Sias and {Del Nonno}, Franca and Andrea Baiocchini and Claudia Cimaglia and Alessandro Agresta and Capobianchi, {Maria Rosaria}",
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T1 - Frequency and multiplicity of human papillomavirus infection in HIV-1 positive women in Italy

AU - Garbuglia, Anna Rosa

AU - Piselli, Pierluca

AU - Lapa, Daniele

AU - Sias, Catia

AU - Del Nonno, Franca

AU - Baiocchini, Andrea

AU - Cimaglia, Claudia

AU - Agresta, Alessandro

AU - Capobianchi, Maria Rosaria

PY - 2012/6

Y1 - 2012/6

N2 - Background: Natural history of HPV infection is altered in HIV positive women, with increased risk of cervical dysplasia. Limited data are available about the influence of HPV genotypes and HPV multiple infections on cervical disease in HIV positive women. Objectives: We determined HPV genotype prevalence in cervical samples from 553 HIV-infected women attending the gynaecological service at " L. Spallanzani" Hospital. Association of HPV multiple infections with cervical abnormalities was investigated. Study design: MY09/MY11 consensus primers were used to detect HPV-DNA; HPV typing was performed by RFLP. A commercial array based kit was used to define unresolved RFLP patterns. Results: HPV was detected in 244/553 (44.1%) patients, correlating with low CD4 counts (p3). Dyskaryosis was associated with decreased CD4 counts (≥500 vs 200-499 vs 3, χ 2 for trend, p=0.001) and with HPV types multiplicity (1 vs 2-3 vs ≥4, χ 2 for trend, p

AB - Background: Natural history of HPV infection is altered in HIV positive women, with increased risk of cervical dysplasia. Limited data are available about the influence of HPV genotypes and HPV multiple infections on cervical disease in HIV positive women. Objectives: We determined HPV genotype prevalence in cervical samples from 553 HIV-infected women attending the gynaecological service at " L. Spallanzani" Hospital. Association of HPV multiple infections with cervical abnormalities was investigated. Study design: MY09/MY11 consensus primers were used to detect HPV-DNA; HPV typing was performed by RFLP. A commercial array based kit was used to define unresolved RFLP patterns. Results: HPV was detected in 244/553 (44.1%) patients, correlating with low CD4 counts (p3). Dyskaryosis was associated with decreased CD4 counts (≥500 vs 200-499 vs 3, χ 2 for trend, p=0.001) and with HPV types multiplicity (1 vs 2-3 vs ≥4, χ 2 for trend, p

KW - Cervical lesions

KW - H-SIL

KW - HIV

KW - HPV multiple infection

KW - HPV prevalence

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