Frequency of donor cytotoxic T cell precursors does not correlate with occurrence of acute graft-versus-host disease in children transplanted using unrelated donors

Daniela Montagna, Rita Maccario, Patrizia Comoli, Luisella Prete, Marco Zecca, Eugenia Giraldi, Cristina Daielli, Antonia Moretta, Piero De Stefano, Franco Locatelli

Research output: Contribution to journalArticlepeer-review

Abstract

Bone marrow transplantation (BMT) from unrelated volunteers is frequently associated with both increased incidence and increased severity of acute graft-versus-host disease (GVHD). In the last years, it has been suggested that the analysis of the frequency of cytotoxic T lymphocyte precursors (CTLp) of unrelated HLA-matched donors can be used to detect disparity for HLA class I antigens and as a predictive test for development of severe GVHD. In this report, we summarized our experience regarding 20 pediatric patients affected by various hematological disorders, receiving allogeneic BMT from unrelated donors. HLA class I and II antigens of donor/recipient pairs were determined by means of serological typing, whereas molecular typing of HLA-class II antigens of patients and their potential donors was performed using PCR-SSP and PCR-fingerprinting techniques. CTLp values, estimated using limiting dilution analysis, were high (range, 1:7000-1:40,000) in 9 of 20 patients, while the other 11 children had low or undetectable levels (

Original languageEnglish
Pages (from-to)107-114
Number of pages8
JournalJournal of Clinical Immunology
Volume16
Issue number2
DOIs
Publication statusPublished - 1996

Keywords

  • Acute graft-versus-host disease
  • Bone marrow transplantation
  • Cytotoxic T lymphocyte precursor frequency
  • Matched unrelated donor

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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