Frequent alterations in the expression of serine/threonine kinases in human cancers

Maria Capra, Paolo Giovanni Nuciforo, Stefano Confalonieri, Micaela Quarto, Marco Bianchi, Manuela Nebuloni, Renzo Boldorini, Francesco Pallotti, Giuseppe Viale, Mikhail L. Gishizky, Giulio F. Draetta, Pier Paolo Di Fiore

Research output: Contribution to journalArticle

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Abstract

Protein kinases constitute a large family of regulatory enzymes involved in the homeostasis of virtually every cellular process. Subversion of protein kinases has been frequently implicated in malignant transformation. Within the family, serine/threonine kinases (STK) have received comparatively lesser attention, vis-a-vis tyrosine kinases, in terms of their involvement in human cancers. Here, we report a large-scale screening of 125 STK, selected to represent all major subgroups within the subfamily, on nine different types of tumors (∼200 patients), by using in situ hybridization on tissue microarrays. Twenty-one STK displayed altered levels of transcripts in tumors, frequently with a clear tumor type-specific dimension. We identified three patterns of alterations in tumors: (a) overexpression in the absence of expression in the normal tissues (10 kinases), (b) overexpression in the presence of expression by normal tissues (8 kinases), and (c) underexpression (3 kinases). Selected members of the three classes were subjected to in-depth analysis on larger case collections and showed significant correlations between their altered expression and biological and/or clinical variables. Our findings suggest that alteration in the expression of STK is a relatively frequent occurrence in human tumors. Among the overexpressed kinases, 10 were undetectable in normal controls and are therefore ideal candidates for further validation as potential targets of molecular cancer therapy.

Original languageEnglish
Pages (from-to)8147-8154
Number of pages8
JournalCancer Research
Volume66
Issue number16
DOIs
Publication statusPublished - Aug 15 2006

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Protein-Serine-Threonine Kinases
Phosphotransferases
Neoplasms
Protein Kinases
Protein-Tyrosine Kinases
In Situ Hybridization
Homeostasis
Enzymes

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Capra, M., Nuciforo, P. G., Confalonieri, S., Quarto, M., Bianchi, M., Nebuloni, M., ... Di Fiore, P. P. (2006). Frequent alterations in the expression of serine/threonine kinases in human cancers. Cancer Research, 66(16), 8147-8154. https://doi.org/10.1158/0008-5472.CAN-05-3489

Frequent alterations in the expression of serine/threonine kinases in human cancers. / Capra, Maria; Nuciforo, Paolo Giovanni; Confalonieri, Stefano; Quarto, Micaela; Bianchi, Marco; Nebuloni, Manuela; Boldorini, Renzo; Pallotti, Francesco; Viale, Giuseppe; Gishizky, Mikhail L.; Draetta, Giulio F.; Di Fiore, Pier Paolo.

In: Cancer Research, Vol. 66, No. 16, 15.08.2006, p. 8147-8154.

Research output: Contribution to journalArticle

Capra, M, Nuciforo, PG, Confalonieri, S, Quarto, M, Bianchi, M, Nebuloni, M, Boldorini, R, Pallotti, F, Viale, G, Gishizky, ML, Draetta, GF & Di Fiore, PP 2006, 'Frequent alterations in the expression of serine/threonine kinases in human cancers', Cancer Research, vol. 66, no. 16, pp. 8147-8154. https://doi.org/10.1158/0008-5472.CAN-05-3489
Capra M, Nuciforo PG, Confalonieri S, Quarto M, Bianchi M, Nebuloni M et al. Frequent alterations in the expression of serine/threonine kinases in human cancers. Cancer Research. 2006 Aug 15;66(16):8147-8154. https://doi.org/10.1158/0008-5472.CAN-05-3489
Capra, Maria ; Nuciforo, Paolo Giovanni ; Confalonieri, Stefano ; Quarto, Micaela ; Bianchi, Marco ; Nebuloni, Manuela ; Boldorini, Renzo ; Pallotti, Francesco ; Viale, Giuseppe ; Gishizky, Mikhail L. ; Draetta, Giulio F. ; Di Fiore, Pier Paolo. / Frequent alterations in the expression of serine/threonine kinases in human cancers. In: Cancer Research. 2006 ; Vol. 66, No. 16. pp. 8147-8154.
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