Frequent coexpression of cytolytic activity and lymphokine production among human T lymphocytes. Production of B cell growth factor and interleukin 2 by T8+ and T4+ cytolytic clones

M. C. Mingari, A. Moretta, E. Maggi, G. Pantaleo, F. Gerosa, S. Romagnani

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

These studies were designed to determine the degree of overlapping between cytolytic function and lymphokine production among peripheral blood human T lymphocytes. T8+ and T4+ cells were obtained by sorting purified T cells using the fluorescence-activated cell sorter (FACS) and cloned at 0.25 cells/well in a microculture system that allows clonal growth of every T cell. 49/49 T8+ clones but only 5/102 T4+ clones displayed cytolytic activity, as assessed by a lectin-dependent assay. Cytolytic clones were further analyzed for their ability to release interleukin 2 (IL 2) and B cell growth factor(s) (BCGF) upon 24 h stimulation with phytohemagglutinin. Fifteen percent and 18% of T8+ cytolytic clones were producing IL 2 were also producing BCGF, 5 clones released either BCGF or IL 2. The present studies indicate that the coexpression of cytolytic and helper/inducer functions is relatively common among peripheral blood T lymphocytes, respectively, irrespective of their T8+ or T4+ phenotype.

Original languageEnglish
Pages (from-to)1066-1069
Number of pages4
JournalEuropean Journal of Immunology
Volume14
Issue number11
DOIs
Publication statusPublished - 1984

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Lymphokines
Interleukin-2
Intercellular Signaling Peptides and Proteins
B-Lymphocytes
Clone Cells
T-Lymphocytes
CD8-Positive T-Lymphocytes
CD4-Positive T-Lymphocytes
Phytohemagglutinins
Lectins
Fluorescence
Phenotype
Growth

ASJC Scopus subject areas

  • Immunology

Cite this

Frequent coexpression of cytolytic activity and lymphokine production among human T lymphocytes. Production of B cell growth factor and interleukin 2 by T8+ and T4+ cytolytic clones. / Mingari, M. C.; Moretta, A.; Maggi, E.; Pantaleo, G.; Gerosa, F.; Romagnani, S.

In: European Journal of Immunology, Vol. 14, No. 11, 1984, p. 1066-1069.

Research output: Contribution to journalArticle

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AU - Maggi, E.

AU - Pantaleo, G.

AU - Gerosa, F.

AU - Romagnani, S.

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