Frequent detection of antiretroviral drug resistance in HIV-1-infected orphaned children followed at a donor-funded rural pediatric clinic in Dodoma, Tanzania

Genny Meini, Maddalena Balestrieri, Sergio Cianchino, Danilo Tacconi, Marco Rossi De Gasperis, Carlo Concato, Ilaria Vicenti, Andrea Rosi, Francesco Saladini, Francesco Callea, Maurizio Zazzi

Research output: Contribution to journalArticle

Abstract

A total of 81 HIV-1 protease (PR) and reverse transcriptase (RT) sequences were obtained from 46 drug-naive and 35 pretreated individual HIV-1-infected orphaned children followed at a donor-funded rural pediatric clinic in Dodoma, Tanzania. PR and RT sequencing was performed by home-brew technology on 70 plasma samples and 11 dried blood spot specimens. Nucleoside RT inhibitor (NRTI) resistance mutations were detected in 2.2% of drug-naive and 82.9% of pretreated children. Nonnucleoside RT inhibitor (NNRTI) resistance mutations were detected in 69.6% of drug-naive and 91.4% of pretreated children. Resistance to protease inhibitors was rare (8.6% in pretreated children). Based on few complete treatment records, only around 20% of the treated children had undetectable plasma HIV-1 RNA. The rate of NRTI and NNRTI resistance in this donor-funded rural pediatric clinic was high and appeared to limit virological response to treatment.

Original languageEnglish
Pages (from-to)448-451
Number of pages4
JournalAIDS Research and Human Retroviruses
Volume31
Issue number4
DOIs
Publication statusPublished - Apr 1 2015

ASJC Scopus subject areas

  • Immunology
  • Virology
  • Infectious Diseases

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    Meini, G., Balestrieri, M., Cianchino, S., Tacconi, D., Rossi De Gasperis, M., Concato, C., Vicenti, I., Rosi, A., Saladini, F., Callea, F., & Zazzi, M. (2015). Frequent detection of antiretroviral drug resistance in HIV-1-infected orphaned children followed at a donor-funded rural pediatric clinic in Dodoma, Tanzania. AIDS Research and Human Retroviruses, 31(4), 448-451. https://doi.org/10.1089/aid.2014.0251