Fresh frozen plasma transfusion in patients with cirrhosis and coagulopathy: Effect on conventional coagulation tests and thrombomodulin-modified thrombin generation

Amanda Bruder Rassi, Elbio Antonio d'Amico, Armando Tripodi, Tânia Rubia Flores da Rocha, Beatriz Yuri Migita, Caroline Marcondes Ferreira, Flair José Carrilho, Alberto Queiroz Farias

Research output: Contribution to journalArticle

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Abstract

Background & Aims: The efficacy of fresh frozen plasma (FFP) transfusion in enhancing thrombin generation in patients with cirrhosis and impaired conventional coagulation tests has not been sufficiently explored. Thus, we aimed to assess the effect of FFP transfusion on thrombin generation in these patients. Methods: Fifty-three consecutive patients receiving a standard dose of FFP to treat bleeding and/or before invasive procedures – if international normalized ratio (INR)/prothrombin time (PT) ratio were ≥1.5 – were prospectively enrolled. The primary endpoint was the amelioration of endogenous thrombin potential (ETP) with thrombomodulin (ETP-TM) after transfusion, which corresponds to the total amount of generated thrombin. INR/PT ratio and activated partial thromboplastin time (aPTT) were also assessed before and after transfusion. Results: FFP enhanced ETP-TM by 5.7%, from 973 (731–1,258) to 1,028 (885–1,343 nM × min; p = 0.019). Before transfusion, evidence of normal or high ETP-TM was found in 94% of patients, even in those with bacterial infections. Only 1 (1.9%) patient had ETP-TM values reverting to the normal range after transfusion. Notably, no patients with low ETP-TM had bleeding. The median decrease in ETP-TM was 8.3% and the mean was 12.8% in 18 (34%) patients after transfusion (from 1,225 [1,071–1,537] to 1,124 [812–1,370] nM × min; p ≤0.0001). Similar responses to FFP transfusion were observed in patients with compensated and acute decompensated cirrhosis, acute-on-chronic liver failure, infection or shock. FFP significantly ameliorated INR and aPTT values (p <0.0001), but in a minority of patients the values were reduced to less than the cut-off point of 1.5. Conclusions: FFP transfusion enhanced thrombin generation and ameliorated conventional coagulation tests to normal values in a limited number of patients, and slightly decreased thrombin generation in 34% of cases. Lay summary: Transfusion of fresh frozen plasma in patients with cirrhosis only slightly improves coagulation test values in a limited number of patients and even appears to worsen them in a third of cases. Transfusion for the purpose of preventing or treating bleeding events could cause inherent risks and costs without clear benefits.

Original languageEnglish
Pages (from-to)85-94
JournalJournal of Hepatology
Volume72
Issue number1
DOIs
Publication statusPublished - 2020

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Thrombomodulin
Thrombin
Fibrosis
International Normalized Ratio
Partial Thromboplastin Time
Prothrombin Time
Hemorrhage
Reference Values
Bacterial Infections
Shock

Keywords

  • Cirrhosis
  • Coagulation
  • Fresh frozen plasma
  • Thrombin generation

ASJC Scopus subject areas

  • Hepatology

Cite this

Fresh frozen plasma transfusion in patients with cirrhosis and coagulopathy : Effect on conventional coagulation tests and thrombomodulin-modified thrombin generation. / Rassi, Amanda Bruder; d'Amico, Elbio Antonio; Tripodi, Armando; da Rocha, Tânia Rubia Flores; Migita, Beatriz Yuri; Ferreira, Caroline Marcondes; Carrilho, Flair José; Farias, Alberto Queiroz.

In: Journal of Hepatology, Vol. 72, No. 1, 2020, p. 85-94.

Research output: Contribution to journalArticle

Rassi, Amanda Bruder ; d'Amico, Elbio Antonio ; Tripodi, Armando ; da Rocha, Tânia Rubia Flores ; Migita, Beatriz Yuri ; Ferreira, Caroline Marcondes ; Carrilho, Flair José ; Farias, Alberto Queiroz. / Fresh frozen plasma transfusion in patients with cirrhosis and coagulopathy : Effect on conventional coagulation tests and thrombomodulin-modified thrombin generation. In: Journal of Hepatology. 2020 ; Vol. 72, No. 1. pp. 85-94.
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T2 - Effect on conventional coagulation tests and thrombomodulin-modified thrombin generation

AU - Rassi, Amanda Bruder

AU - d'Amico, Elbio Antonio

AU - Tripodi, Armando

AU - da Rocha, Tânia Rubia Flores

AU - Migita, Beatriz Yuri

AU - Ferreira, Caroline Marcondes

AU - Carrilho, Flair José

AU - Farias, Alberto Queiroz

PY - 2020

Y1 - 2020

N2 - Background & Aims: The efficacy of fresh frozen plasma (FFP) transfusion in enhancing thrombin generation in patients with cirrhosis and impaired conventional coagulation tests has not been sufficiently explored. Thus, we aimed to assess the effect of FFP transfusion on thrombin generation in these patients. Methods: Fifty-three consecutive patients receiving a standard dose of FFP to treat bleeding and/or before invasive procedures – if international normalized ratio (INR)/prothrombin time (PT) ratio were ≥1.5 – were prospectively enrolled. The primary endpoint was the amelioration of endogenous thrombin potential (ETP) with thrombomodulin (ETP-TM) after transfusion, which corresponds to the total amount of generated thrombin. INR/PT ratio and activated partial thromboplastin time (aPTT) were also assessed before and after transfusion. Results: FFP enhanced ETP-TM by 5.7%, from 973 (731–1,258) to 1,028 (885–1,343 nM × min; p = 0.019). Before transfusion, evidence of normal or high ETP-TM was found in 94% of patients, even in those with bacterial infections. Only 1 (1.9%) patient had ETP-TM values reverting to the normal range after transfusion. Notably, no patients with low ETP-TM had bleeding. The median decrease in ETP-TM was 8.3% and the mean was 12.8% in 18 (34%) patients after transfusion (from 1,225 [1,071–1,537] to 1,124 [812–1,370] nM × min; p ≤0.0001). Similar responses to FFP transfusion were observed in patients with compensated and acute decompensated cirrhosis, acute-on-chronic liver failure, infection or shock. FFP significantly ameliorated INR and aPTT values (p <0.0001), but in a minority of patients the values were reduced to less than the cut-off point of 1.5. Conclusions: FFP transfusion enhanced thrombin generation and ameliorated conventional coagulation tests to normal values in a limited number of patients, and slightly decreased thrombin generation in 34% of cases. Lay summary: Transfusion of fresh frozen plasma in patients with cirrhosis only slightly improves coagulation test values in a limited number of patients and even appears to worsen them in a third of cases. Transfusion for the purpose of preventing or treating bleeding events could cause inherent risks and costs without clear benefits.

AB - Background & Aims: The efficacy of fresh frozen plasma (FFP) transfusion in enhancing thrombin generation in patients with cirrhosis and impaired conventional coagulation tests has not been sufficiently explored. Thus, we aimed to assess the effect of FFP transfusion on thrombin generation in these patients. Methods: Fifty-three consecutive patients receiving a standard dose of FFP to treat bleeding and/or before invasive procedures – if international normalized ratio (INR)/prothrombin time (PT) ratio were ≥1.5 – were prospectively enrolled. The primary endpoint was the amelioration of endogenous thrombin potential (ETP) with thrombomodulin (ETP-TM) after transfusion, which corresponds to the total amount of generated thrombin. INR/PT ratio and activated partial thromboplastin time (aPTT) were also assessed before and after transfusion. Results: FFP enhanced ETP-TM by 5.7%, from 973 (731–1,258) to 1,028 (885–1,343 nM × min; p = 0.019). Before transfusion, evidence of normal or high ETP-TM was found in 94% of patients, even in those with bacterial infections. Only 1 (1.9%) patient had ETP-TM values reverting to the normal range after transfusion. Notably, no patients with low ETP-TM had bleeding. The median decrease in ETP-TM was 8.3% and the mean was 12.8% in 18 (34%) patients after transfusion (from 1,225 [1,071–1,537] to 1,124 [812–1,370] nM × min; p ≤0.0001). Similar responses to FFP transfusion were observed in patients with compensated and acute decompensated cirrhosis, acute-on-chronic liver failure, infection or shock. FFP significantly ameliorated INR and aPTT values (p <0.0001), but in a minority of patients the values were reduced to less than the cut-off point of 1.5. Conclusions: FFP transfusion enhanced thrombin generation and ameliorated conventional coagulation tests to normal values in a limited number of patients, and slightly decreased thrombin generation in 34% of cases. Lay summary: Transfusion of fresh frozen plasma in patients with cirrhosis only slightly improves coagulation test values in a limited number of patients and even appears to worsen them in a third of cases. Transfusion for the purpose of preventing or treating bleeding events could cause inherent risks and costs without clear benefits.

KW - Cirrhosis

KW - Coagulation

KW - Fresh frozen plasma

KW - Thrombin generation

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