FRET microscopy autologous tumor lysate processing in mature dendritic cell vaccine therapy

Laura Fiammenghi, Valentina Ancarani, Tilman Rosales, Jay R. Knutson, Massimiliano Petrini, Anna M. Granato, Elena Pancisi, Laura Ridolfi, Ruggero Ridolfi, Angela Riccobon, Paolo Neyroz

Research output: Contribution to journalArticle

Abstract

Background: Antigen processing by dendritic cells (DC) exposed to specific stimuli has been well characterized in biological studies. Nonetheless, the question of whether autologous whole tumor lysates (as used in clinical trials) are similarly processed by these cells has not yet been resolved.Methods: In this study, we examined the transfer of peptides from whole tumor lysates to major histocompatibility complex class II molecules (MHC II) in mature dendritic cells (mDC) from a patient with advanced melanoma. Tumor antigenic peptides-MHC II proximity was revealed by Förster Resonance Energy Transfer (FRET) measurements, which effectively extends the application of fluorescence microscopy to the molecular level (

Original languageEnglish
Article number52
JournalJournal of Translational Medicine
Volume8
DOIs
Publication statusPublished - Jun 3 2010

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

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  • Cite this

    Fiammenghi, L., Ancarani, V., Rosales, T., Knutson, J. R., Petrini, M., Granato, A. M., Pancisi, E., Ridolfi, L., Ridolfi, R., Riccobon, A., & Neyroz, P. (2010). FRET microscopy autologous tumor lysate processing in mature dendritic cell vaccine therapy. Journal of Translational Medicine, 8, [52]. https://doi.org/10.1186/1479-5876-8-52