FRETS-VWF73 rather than CBA assay reflects ADAMTS13 proteolytic activity in acquired thrombotic thrombocytopenic purpura patients

Ilaria Mancini; Carla Valsecchi; Luca Andrea Lotta; Louis Deforche; Silvia Pontiggia; Mariateresa Bajetta; Roberta Palla; Karen Vanhoorelbeke; Flora Peyvandi

doi:10.1160/TH13-08-0688

FRETS-VWF73 rather than CBA assay reflects ADAMTS13 proteolytic activity in acquired thrombotic thrombocytopenic purpura patients

Ilaria Mancini, Carla Valsecchi, Luca Andrea Lotta, Louis Deforche, Silvia Pontiggia, Mariateresa Bajetta, Roberta Palla, Karen Vanhoorelbeke, Flora Peyvandi

Fondazione IRCCS Ca' Granda- Ospedale Maggiore Policlinico

Research output: Contribution to journal › Article › peer-review

Abstract

Collagen-binding activity (CBA) and FRETS-VWF73 assays are widely adopted methods for the measurement of the plasmatic activity of ADAMTS13, the von Willebrand factor (VWF) cleaving-protease. Accurately assessing the severe deficiency of ADAMTS13 is important in the management of thrombotic thrombocytopenic purpura (TTP). However, non-concordant results between the two assays have been reported in a small but relevant percentage of TTP cases. We investigated whether CBA or FRETS-VWF73 assay reflects ADAMTS13 proteolytic activity in acquired TTP patients with non-concordant measurements. Twenty plasma samples with non-concordant ADAMTS13 activity results,

Original language	English
Pages (from-to)	297-303
Number of pages	7
Journal	Thrombosis and Haemostasis
Volume	112
Issue number	2
DOIs	https://doi.org/10.1160/TH13-08-0688
Publication status	Published - 2014

Keywords

ADAMTS13 activity assay
Autoantibodies
Flow assay
TTP
Von Willebrand factor

ASJC Scopus subject areas

Hematology
Medicine(all)

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FRETS-VWF73 rather than CBA assay reflects ADAMTS13 proteolytic activity in acquired thrombotic thrombocytopenic purpura patients. / Mancini, Ilaria; Valsecchi, Carla; Lotta, Luca Andrea; Deforche, Louis; Pontiggia, Silvia; Bajetta, Mariateresa; Palla, Roberta; Vanhoorelbeke, Karen; Peyvandi, Flora.

In: Thrombosis and Haemostasis, Vol. 112, No. 2, 2014, p. 297-303.

Research output: Contribution to journal › Article › peer-review

Mancini, Ilaria ; Valsecchi, Carla ; Lotta, Luca Andrea ; Deforche, Louis ; Pontiggia, Silvia ; Bajetta, Mariateresa ; Palla, Roberta ; Vanhoorelbeke, Karen ; Peyvandi, Flora. / FRETS-VWF73 rather than CBA assay reflects ADAMTS13 proteolytic activity in acquired thrombotic thrombocytopenic purpura patients. In: Thrombosis and Haemostasis. 2014 ; Vol. 112, No. 2. pp. 297-303.

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abstract = "Collagen-binding activity (CBA) and FRETS-VWF73 assays are widely adopted methods for the measurement of the plasmatic activity of ADAMTS13, the von Willebrand factor (VWF) cleaving-protease. Accurately assessing the severe deficiency of ADAMTS13 is important in the management of thrombotic thrombocytopenic purpura (TTP). However, non-concordant results between the two assays have been reported in a small but relevant percentage of TTP cases. We investigated whether CBA or FRETS-VWF73 assay reflects ADAMTS13 proteolytic activity in acquired TTP patients with non-concordant measurements. Twenty plasma samples with non-concordant ADAMTS13 activity results, ",

keywords = "ADAMTS13 activity assay, Autoantibodies, Flow assay, TTP, Von Willebrand factor",

author = "Ilaria Mancini and Carla Valsecchi and Lotta, {Luca Andrea} and Louis Deforche and Silvia Pontiggia and Mariateresa Bajetta and Roberta Palla and Karen Vanhoorelbeke and Flora Peyvandi",

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AU - Mancini, Ilaria

AU - Valsecchi, Carla

AU - Lotta, Luca Andrea

AU - Deforche, Louis

AU - Pontiggia, Silvia

AU - Bajetta, Mariateresa

AU - Palla, Roberta

AU - Vanhoorelbeke, Karen

AU - Peyvandi, Flora

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AB - Collagen-binding activity (CBA) and FRETS-VWF73 assays are widely adopted methods for the measurement of the plasmatic activity of ADAMTS13, the von Willebrand factor (VWF) cleaving-protease. Accurately assessing the severe deficiency of ADAMTS13 is important in the management of thrombotic thrombocytopenic purpura (TTP). However, non-concordant results between the two assays have been reported in a small but relevant percentage of TTP cases. We investigated whether CBA or FRETS-VWF73 assay reflects ADAMTS13 proteolytic activity in acquired TTP patients with non-concordant measurements. Twenty plasma samples with non-concordant ADAMTS13 activity results,

KW - ADAMTS13 activity assay

KW - Autoantibodies

KW - Flow assay

KW - TTP

KW - Von Willebrand factor

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FRETS-VWF73 rather than CBA assay reflects ADAMTS13 proteolytic activity in acquired thrombotic thrombocytopenic purpura patients

Abstract

Keywords

ASJC Scopus subject areas

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