TY - JOUR
T1 - FRETS-VWF73 rather than CBA assay reflects ADAMTS13 proteolytic activity in acquired thrombotic thrombocytopenic purpura patients
AU - Mancini, Ilaria
AU - Valsecchi, Carla
AU - Lotta, Luca Andrea
AU - Deforche, Louis
AU - Pontiggia, Silvia
AU - Bajetta, Mariateresa
AU - Palla, Roberta
AU - Vanhoorelbeke, Karen
AU - Peyvandi, Flora
PY - 2014
Y1 - 2014
N2 - Collagen-binding activity (CBA) and FRETS-VWF73 assays are widely adopted methods for the measurement of the plasmatic activity of ADAMTS13, the von Willebrand factor (VWF) cleaving-protease. Accurately assessing the severe deficiency of ADAMTS13 is important in the management of thrombotic thrombocytopenic purpura (TTP). However, non-concordant results between the two assays have been reported in a small but relevant percentage of TTP cases. We investigated whether CBA or FRETS-VWF73 assay reflects ADAMTS13 proteolytic activity in acquired TTP patients with non-concordant measurements. Twenty plasma samples with non-concordant ADAMTS13 activity results,
AB - Collagen-binding activity (CBA) and FRETS-VWF73 assays are widely adopted methods for the measurement of the plasmatic activity of ADAMTS13, the von Willebrand factor (VWF) cleaving-protease. Accurately assessing the severe deficiency of ADAMTS13 is important in the management of thrombotic thrombocytopenic purpura (TTP). However, non-concordant results between the two assays have been reported in a small but relevant percentage of TTP cases. We investigated whether CBA or FRETS-VWF73 assay reflects ADAMTS13 proteolytic activity in acquired TTP patients with non-concordant measurements. Twenty plasma samples with non-concordant ADAMTS13 activity results,
KW - ADAMTS13 activity assay
KW - Autoantibodies
KW - Flow assay
KW - TTP
KW - Von Willebrand factor
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U2 - 10.1160/TH13-08-0688
DO - 10.1160/TH13-08-0688
M3 - Article
C2 - 24740645
AN - SCOPUS:84905176550
VL - 112
SP - 297
EP - 303
JO - Thrombosis and Haemostasis
JF - Thrombosis and Haemostasis
SN - 0340-6245
IS - 2
ER -