Abstract
The identification of molecules involved in tumor initiation and progression is fundamental for understanding disease’s biology and, as a consequence, for the clinical management of patients. In the present work we will describe an optimized proteomic approach for the identification of molecules involved in the progression of Chronic Lymphocytic Leukemia (CLL). In detail, leukemic cell lysates are resolved by 2-dimensional Electrophoresis (2DE) and visualized as “spots” on the 2DE gels. Comparative analysis of proteomic maps allows the identification of differentially expressed proteins (in terms of abundance and post-translational modifications) that are picked, isolated and identified by Mass Spectrometry (MS). The biological function of the identified candidates can be tested by different assays (i.e. migration, adhesion and F-actin polymerization), that we have optimized for primary leukemic cells.
Original language | English |
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Article number | e51942 |
Journal | Journal of visualized experiments : JoVE |
Issue number | 92 |
DOIs | |
Publication status | Published - Oct 19 2014 |
Keywords
- 2D Electrophoresis
- Chronic lymphocytic leukemia
- Cytoskeleton
- Issue 92
- Lymphocytes
- Mass spectrometry
- Medicine
- Migration
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Chemical Engineering(all)
- Immunology and Microbiology(all)
- Neuroscience(all)
- Medicine(all)