From cell lines to pluripotent stem cells for modelling Parkinson's Disease

Elena Ferrari, Antonella Cardinale, Barbara Picconi, Fabrizio Gardoni

Research output: Contribution to journalReview articlepeer-review

Abstract

Parkinson's disease (PD) is the second most common neurodegenerative disorder characterized by loss of dopaminergic (DAergic) neurons in the substantia nigra (SN) that contributes to the main motor symptoms of the disease. At present, even if several advancements have been done in the last decades, the molecular and cellular mechanisms involved in the pathogenesis are far to be fully understood. Accordingly, the establishment of reliable in vitro experimental models to investigate the early events of the pathogenesis represents a key issue in the field. However, to mimic and reproduce in vitro the complex neuronal circuitry involved in PD-associated degeneration of DAergic neurons still remains a highly challenging issue. Here we will review the in vitro PD models used in the last 25 years of research, ranging from cell lines, primary rat or mice neuronal cultures to the more recent use of human induced pluripotent stem cells (hiPSCs) and, finally, the development of 3D midbrain organoids.

Original languageEnglish
Article number108741
Pages (from-to)1-10
Number of pages10
JournalJournal of Neuroscience Methods
Volume340
DOIs
Publication statusPublished - Jul 1 2020

Keywords

  • cell cultures
  • dopamine
  • glutamate
  • in vitro models
  • striatum

ASJC Scopus subject areas

  • Neuroscience(all)

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