From human Megakaryocytes to platelets: Effects of aspirin on high-mobility group Box 1/receptor for advanced glycation end products axis

S Mardente, Emanuela Mari, I Massimi, M Tafani, R Guerriero, O Morsilli, FM Pulcinelli, ME Bianchi, Alessandra Zicari

Research output: Contribution to journalArticle

Abstract

Platelets (PLTs) are the major source of high-mobility group box 1 (HMGB1), a protein that is involved in sterile inflammation of blood vessels and thrombosis. Megakaryocytes (MKs) synthesize HMGB1 and transfer both protein and mRNA into PLTs and PLT-derived microvesicles (MV). Free HMGB1 found in supernatants of in vitro differentiated MKs and in a megakaryoblastic cell line (DAMI cells). Aspirin "in vivo" and "in vitro" not only reduces HMGB1 and receptor for advanced glycation end products expression on MKs and PLTs but also drives the movement of HMGB1 from MKs into PLTs and PLT-derived MV. These findings suggest that consumption of low doses of aspirin reduces the risk of atherosclerosis complications as well as reducing PLT aggregation by the inhibition of COX-1. © 2018 Mardente, Mari, Massimi, Tafani, Guerriero, Morsilli, Pulcinelli, Bianchi and Zicari.
Original languageEnglish
Article number1946
JournalFrontiers in Immunology
Volume8
DOIs
Publication statusPublished - 2018

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Megakaryocytes
Aspirin
Blood Platelets
HMGB1 Protein
Platelet Aggregation
Blood Vessels
Advanced Glycosylation End Product-Specific Receptor
Atherosclerosis
Thrombosis
Inflammation
Cell Line
Messenger RNA
Proteins

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From human Megakaryocytes to platelets: Effects of aspirin on high-mobility group Box 1/receptor for advanced glycation end products axis. / Mardente, S; Mari, Emanuela; Massimi, I; Tafani, M; Guerriero, R; Morsilli, O; Pulcinelli, FM; Bianchi, ME; Zicari, Alessandra.

In: Frontiers in Immunology, Vol. 8, 1946, 2018.

Research output: Contribution to journalArticle

Mardente, S ; Mari, Emanuela ; Massimi, I ; Tafani, M ; Guerriero, R ; Morsilli, O ; Pulcinelli, FM ; Bianchi, ME ; Zicari, Alessandra. / From human Megakaryocytes to platelets: Effects of aspirin on high-mobility group Box 1/receptor for advanced glycation end products axis. In: Frontiers in Immunology. 2018 ; Vol. 8.
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AU - Massimi, I

AU - Tafani, M

AU - Guerriero, R

AU - Morsilli, O

AU - Pulcinelli, FM

AU - Bianchi, ME

AU - Zicari, Alessandra

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AB - Platelets (PLTs) are the major source of high-mobility group box 1 (HMGB1), a protein that is involved in sterile inflammation of blood vessels and thrombosis. Megakaryocytes (MKs) synthesize HMGB1 and transfer both protein and mRNA into PLTs and PLT-derived microvesicles (MV). Free HMGB1 found in supernatants of in vitro differentiated MKs and in a megakaryoblastic cell line (DAMI cells). Aspirin "in vivo" and "in vitro" not only reduces HMGB1 and receptor for advanced glycation end products expression on MKs and PLTs but also drives the movement of HMGB1 from MKs into PLTs and PLT-derived MV. These findings suggest that consumption of low doses of aspirin reduces the risk of atherosclerosis complications as well as reducing PLT aggregation by the inhibition of COX-1. © 2018 Mardente, Mari, Massimi, Tafani, Guerriero, Morsilli, Pulcinelli, Bianchi and Zicari.

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