From hundreds to thousands: Widening the normal human Urinome

Laura Santucci, Giovanni Candiano, Andrea Petretto, Maurizio Bruschi, Chiara Lavarello, Elvira Inglese, Pier Giorgio Righetti, Gian Marco Ghiggeri

Research output: Contribution to journalArticle

Abstract

The limits on protein detection in urine are unknown. Improving the analytical approach to detection would increase the number of identified proteins and potentially strengthen their predictive potential in diseases. Here, we present the data that resulted from a combination of analytical procedures for maximizing sensitivity and reproducibility of normal human urinary proteome analysis. These procedures are ultracentrifugation, vesicle separation, combinatorial peptide ligand libraries (CPLL) and solvent removal of pigments. Proteins were identified by an Orbitrap Velos Mass Spectrometry. 3429 proteins are characterized, 1724 of which are novel discoveries. The data are related to Santucci et al. (in press) [1] and available both here and at ChorusProject.org under project name "From hundreds to thousands: widening the normal human Urinome". The material supplied to Chorus Progect.org includes technical MS spectra data only.

Original languageEnglish
Pages (from-to)25-28
Number of pages4
JournalData in Brief
Volume1
DOIs
Publication statusPublished - Dec 1 2014

Keywords

  • Combinatorial peptide ligand libraries
  • Mass spectrometry
  • Urinary proteome
  • Vesicles

ASJC Scopus subject areas

  • General
  • Education

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