Frontline Science: Mast cells regulate neutrophil homeostasis by influencing macrophage clearance activity

Elena Jachetti, Federica D'Incà, Luca Danelli, Raffaella Magris, Chiara Dal Secco, Filippo Vit, Valeria Cancila, Claudio Tripodo, Patrizia Scapini, Mario Paolo Colombo, Carlo Pucillo, Barbara Frossi

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The receptor tyrosine kinase cKit and its ligand stem cell factor are essential for mast cells (MC) development and survival. Strains with mutations affecting the Kit gene display a profound MC deficiency in all tissues and have been extensively used to investigate the role of MC in both physiologic and pathologic conditions. However, these mice present a variety of abnormalities in other immune cell populations that can affect the interpretation of MC-related responses. C57BL/6 Kit W-sh are characterized by an aberrant extramedullary myelopoiesis and systemic neutrophilia. MC deficiency in Kit W-sh mice can be selectively repaired by engraftment with in vitro-differentiated MC to validate MC-specific functions. Nevertheless, the impact of MC reconstitution on other immune populations has never been evaluated in detail. Here, we specifically investigated the neutrophil compartment in primary and secondary lymphoid organs of C57BL/6 Kit W-sh mice before and after MC reconstitution. We found that, albeit not apparently affecting neutrophils phenotype or maturation, MC reconstitution of Kit W-sh mice restored the number of neutrophils at a level similar to that of wild-type C57BL/6 mice. In vitro and ex vivo experiments indicated that MC can influence neutrophil clearance by increasing macrophages’ phagocytic activity. Furthermore, the G-CSF/IL-17 axis was also influenced by the presence or absence of MC in Kit W-sh mice. These data suggest that MC play a role in the control of neutrophil homeostasis and that this aspect should be taken into account in the interpretation of results obtained using Kit W-sh mice.

Original languageEnglish
Pages (from-to)633-644
Number of pages12
JournalJournal of Leukocyte Biology
Volume105
Issue number4
DOIs
Publication statusPublished - Apr 2019

Fingerprint

Mast Cells
Neutrophils
Homeostasis
Macrophages
Myelopoiesis
Stem Cell Factor
Interleukin-17
Receptor Protein-Tyrosine Kinases
Granulocyte Colony-Stimulating Factor
Inbred C57BL Mouse
Population
Cell Survival
Ligands
Phenotype

Keywords

  • Kit (W-sh) mice
  • macrophages
  • mast cell
  • neutrophils
  • phagocytosis

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Cell Biology

Cite this

Frontline Science : Mast cells regulate neutrophil homeostasis by influencing macrophage clearance activity. / Jachetti, Elena; D'Incà, Federica; Danelli, Luca; Magris, Raffaella; Dal Secco, Chiara; Vit, Filippo; Cancila, Valeria; Tripodo, Claudio; Scapini, Patrizia; Colombo, Mario Paolo; Pucillo, Carlo; Frossi, Barbara.

In: Journal of Leukocyte Biology, Vol. 105, No. 4, 04.2019, p. 633-644.

Research output: Contribution to journalArticle

Jachetti, E, D'Incà, F, Danelli, L, Magris, R, Dal Secco, C, Vit, F, Cancila, V, Tripodo, C, Scapini, P, Colombo, MP, Pucillo, C & Frossi, B 2019, 'Frontline Science: Mast cells regulate neutrophil homeostasis by influencing macrophage clearance activity', Journal of Leukocyte Biology, vol. 105, no. 4, pp. 633-644. https://doi.org/10.1002/JLB.4HI1018-390R
Jachetti, Elena ; D'Incà, Federica ; Danelli, Luca ; Magris, Raffaella ; Dal Secco, Chiara ; Vit, Filippo ; Cancila, Valeria ; Tripodo, Claudio ; Scapini, Patrizia ; Colombo, Mario Paolo ; Pucillo, Carlo ; Frossi, Barbara. / Frontline Science : Mast cells regulate neutrophil homeostasis by influencing macrophage clearance activity. In: Journal of Leukocyte Biology. 2019 ; Vol. 105, No. 4. pp. 633-644.
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