FTIR spectral signatures of mouse antral oocytes: Molecular markers of oocyte maturation and developmental competence

Diletta Ami, Paolo Mereghetti, Antonino Natalello, Silvia Maria Doglia, Mario Zanoni, Carlo Alberto Redi, Manuela Monti

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Mammalian antral oocytes with a Hoescht-positive DNA ring around the nucleolus (SN) are able to resume meiosis and to fully support the embryonic development, while oocytes with a non-surrounded nucleolus (NSN) cannot. Here, we applied FTIR microspectroscopy to characterize single SN and NSN mouse oocytes in order to try to elucidate some aspects of the mechanisms behind the different chromatin organization that impairs the full development of NSN oocyte-derived embryos. To this aim, oocytes were measured at three different stages of their maturation: just after isolation and classification as SN and NSN oocytes (time 0); after 10h of in vitro maturation, i.e. at the completion of the metaphase I (time 1); and after 20h of in vitro maturation, i.e. at the completion of the metaphase II (time 2). Significant spectral differences in the lipid (3050-2800cm-1) and protein (1700-1600cm-1) absorption regions were found between the two types of oocytes and among the different stages of maturation within the same oocyte type. Moreover, dramatic changes in nucleic acid content, concerning mainly the extent of transcription and polyadenylation, were detected in particular between 1000 and 800cm-1. The use of the multivariate principal component-linear discriminant analysis (PCA-LDA) enabled us to identify the maturation stage in which the separation between the two types of oocytes took place, finding as the most discriminating wavenumbers those associated to transcriptional activity and polyadenylation, in agreement with the visual analysis of the spectral data.

Original languageEnglish
Pages (from-to)1220-1229
Number of pages10
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Volume1813
Issue number6
DOIs
Publication statusPublished - Jun 2011

Fingerprint

Fourier Transform Infrared Spectroscopy
Mental Competency
Oocytes
Polyadenylation
Metaphase
Antral
Meiosis
Discriminant Analysis
Nucleic Acids
Chromatin
Embryonic Development
Embryonic Structures
Lipids
DNA

Keywords

  • Developmental competence
  • FTIR microspectroscopy
  • NSN oocytes
  • Oocyte maturation
  • Polyadenylation
  • SN oocytes

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

FTIR spectral signatures of mouse antral oocytes : Molecular markers of oocyte maturation and developmental competence. / Ami, Diletta; Mereghetti, Paolo; Natalello, Antonino; Doglia, Silvia Maria; Zanoni, Mario; Redi, Carlo Alberto; Monti, Manuela.

In: Biochimica et Biophysica Acta - Molecular Cell Research, Vol. 1813, No. 6, 06.2011, p. 1220-1229.

Research output: Contribution to journalArticle

Ami, Diletta ; Mereghetti, Paolo ; Natalello, Antonino ; Doglia, Silvia Maria ; Zanoni, Mario ; Redi, Carlo Alberto ; Monti, Manuela. / FTIR spectral signatures of mouse antral oocytes : Molecular markers of oocyte maturation and developmental competence. In: Biochimica et Biophysica Acta - Molecular Cell Research. 2011 ; Vol. 1813, No. 6. pp. 1220-1229.
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