Fugu intron oversize reveals the presence of U15 snoRNA coding sequences in some introns of the ribosomal protein S3 gene

Claudia Crosio, Francesco Cecconi, Paolo Mariottini, Gianni Cesareni, Sydney Brenner, Francesco Amaldi

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We present here the analysis of the genomic organization of the Fugu gene coding for ribosomal protein S3 and its intron encoded U15 RNA, and compare it with the homologous human and Xenopus genes. Only two of the six Fugu S3 gene introns do not contain the U15 sequence and are in fact shorter than 100 nucleotides, as most Fugu introns. The other four introns are somewhat longer and contain sequences homologous to U15 RNA; two of these represent functional copies, as shown by microinjections of Fugu transcripts into Xenopus oocytes, whereas the other two appear to be nonfunctional pseudocopies. Thus Fugu turns out to be ideal for the study of intron encoded snoRNAs, partly because of the reduced cloning and sequencing workload, and partly because the intron length per se can be an indication of the presence of a snoRNA coding sequence.

Original languageEnglish
Pages (from-to)1227-1231
Number of pages5
JournalGenome Research
Volume6
Issue number12
Publication statusPublished - 1996

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Takifugu
Small Nucleolar RNA
Introns
Genes
Xenopus
RNA
Microinjections
Sequence Homology
Workload
Oocytes
ribosomal protein S3
Organism Cloning
Nucleotides

ASJC Scopus subject areas

  • Genetics

Cite this

Fugu intron oversize reveals the presence of U15 snoRNA coding sequences in some introns of the ribosomal protein S3 gene. / Crosio, Claudia; Cecconi, Francesco; Mariottini, Paolo; Cesareni, Gianni; Brenner, Sydney; Amaldi, Francesco.

In: Genome Research, Vol. 6, No. 12, 1996, p. 1227-1231.

Research output: Contribution to journalArticle

Crosio, Claudia ; Cecconi, Francesco ; Mariottini, Paolo ; Cesareni, Gianni ; Brenner, Sydney ; Amaldi, Francesco. / Fugu intron oversize reveals the presence of U15 snoRNA coding sequences in some introns of the ribosomal protein S3 gene. In: Genome Research. 1996 ; Vol. 6, No. 12. pp. 1227-1231.
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