TY - JOUR
T1 - Full length α-synuclein is present in cerebrospinal fluid from Parkinson's disease and normal subjects
AU - Borghi, Roberta
AU - Marchese, Roberta
AU - Negro, Alessandro
AU - Marinelli, Lucio
AU - Forloni, Gianluigi
AU - Zaccheo, Damiano
AU - Abbruzzese, Giovanni
AU - Tabaton, Massimo
PY - 2000/6/16
Y1 - 2000/6/16
N2 - Several clues suggest that α-synuclein, a presynaptic protein, plays a central role in the pathogenesis of idiopathic Parkinson's disease (PD). To search a peripheral marker of PD, we analyzed presence and amount of α- synuclein in CSF from 12 PD patients and 10 neurologically normal subjects. The protein was extracted from CSF samples through immunoprecipitation and immunoblotting with different specific anti-α-synuclein antibodies. We identified a 19 kDa band that corresponds to monomeric α-synuclein, given its comigration with homologue human recombinant peptide as well as with the protein extracted from cerebral cortex of normal subjects. The amount of CSF 19 kDa α-synuclein did not significantly vary in PD and normal cases. These findings have two implications: (a) full length α-synuclein is released by neurons in the extracellular space; (b) α-synuclein does not appear a peripheral marker of PD pathology. (C) 2000 Elsevier Science Ireland Ltd.
AB - Several clues suggest that α-synuclein, a presynaptic protein, plays a central role in the pathogenesis of idiopathic Parkinson's disease (PD). To search a peripheral marker of PD, we analyzed presence and amount of α- synuclein in CSF from 12 PD patients and 10 neurologically normal subjects. The protein was extracted from CSF samples through immunoprecipitation and immunoblotting with different specific anti-α-synuclein antibodies. We identified a 19 kDa band that corresponds to monomeric α-synuclein, given its comigration with homologue human recombinant peptide as well as with the protein extracted from cerebral cortex of normal subjects. The amount of CSF 19 kDa α-synuclein did not significantly vary in PD and normal cases. These findings have two implications: (a) full length α-synuclein is released by neurons in the extracellular space; (b) α-synuclein does not appear a peripheral marker of PD pathology. (C) 2000 Elsevier Science Ireland Ltd.
KW - α-Synuclein
KW - Biological markers
KW - Cerebrospinal fluid
KW - Neurodegenerative diseases
KW - Parkinson's disease
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U2 - 10.1016/S0304-3940(00)01153-8
DO - 10.1016/S0304-3940(00)01153-8
M3 - Article
C2 - 10841992
AN - SCOPUS:0034674376
VL - 287
SP - 65
EP - 67
JO - Neuroscience Letters
JF - Neuroscience Letters
SN - 0304-3940
IS - 1
ER -