Full length α-synuclein is present in cerebrospinal fluid from Parkinson's disease and normal subjects

Roberta Borghi, Roberta Marchese, Alessandro Negro, Lucio Marinelli, Gianluigi Forloni, Damiano Zaccheo, Giovanni Abbruzzese, Massimo Tabaton

Research output: Contribution to journalArticlepeer-review

Abstract

Several clues suggest that α-synuclein, a presynaptic protein, plays a central role in the pathogenesis of idiopathic Parkinson's disease (PD). To search a peripheral marker of PD, we analyzed presence and amount of α- synuclein in CSF from 12 PD patients and 10 neurologically normal subjects. The protein was extracted from CSF samples through immunoprecipitation and immunoblotting with different specific anti-α-synuclein antibodies. We identified a 19 kDa band that corresponds to monomeric α-synuclein, given its comigration with homologue human recombinant peptide as well as with the protein extracted from cerebral cortex of normal subjects. The amount of CSF 19 kDa α-synuclein did not significantly vary in PD and normal cases. These findings have two implications: (a) full length α-synuclein is released by neurons in the extracellular space; (b) α-synuclein does not appear a peripheral marker of PD pathology. (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish
Pages (from-to)65-67
Number of pages3
JournalNeuroscience Letters
Volume287
Issue number1
DOIs
Publication statusPublished - Jun 16 2000

Keywords

  • α-Synuclein
  • Biological markers
  • Cerebrospinal fluid
  • Neurodegenerative diseases
  • Parkinson's disease

ASJC Scopus subject areas

  • Neuroscience(all)

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