TY - JOUR
T1 - Full-length and N-terminally truncated chicken intestinal diazepam- binding inhibitor. Purification, structural characterization and influence on insulin release
AU - Chen, Zheng Wang
AU - Bergman, Tomas
AU - Jörnvall, Hans
AU - Bonetto, Valentina
AU - Norberg, Åke
AU - Mutt, Viktor
AU - Longone, Patrizia
AU - Costa, Erminio
AU - Efendic, Suad
AU - Östenson, Claes Göran
PY - 1997/3/26
Y1 - 1997/3/26
N2 - Two forms of diazepam-binding inhibitor (DBI) have been purified from chicken intestine and identified as the intact avian polypeptide (residues 1- 86) and a truncated variant (residues 35-86). At 10 nM concentration, both the intact and the truncated peptide suppress in vitro-monitored glucose induced insulin release by 50 (p <0.02) and 64% (p <0.01), respectively. The truncation starts at a segment, -Thr-Val-Gly-Asp-, that is strictly conserved between characterized DBI species, indicating special restrictions on the structure. However, overall DBI conservation appears to be complex. A number of differently bioactive fragments with separate processings and tissue distributions have been observed, suggesting multiple functions of DBI and its sub-segments.
AB - Two forms of diazepam-binding inhibitor (DBI) have been purified from chicken intestine and identified as the intact avian polypeptide (residues 1- 86) and a truncated variant (residues 35-86). At 10 nM concentration, both the intact and the truncated peptide suppress in vitro-monitored glucose induced insulin release by 50 (p <0.02) and 64% (p <0.01), respectively. The truncation starts at a segment, -Thr-Val-Gly-Asp-, that is strictly conserved between characterized DBI species, indicating special restrictions on the structure. However, overall DBI conservation appears to be complex. A number of differently bioactive fragments with separate processings and tissue distributions have been observed, suggesting multiple functions of DBI and its sub-segments.
KW - Avian DBI
KW - Insulin release
KW - Peptide multiplicity
KW - Polymer
KW - Processed peptide
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U2 - 10.1016/S0167-0115(97)02126-5
DO - 10.1016/S0167-0115(97)02126-5
M3 - Article
C2 - 9178347
AN - SCOPUS:0030946579
VL - 69
SP - 63
EP - 68
JO - Regulatory Peptides
JF - Regulatory Peptides
SN - 0167-0115
IS - 2
ER -