Fully reversible procedure for silver staining improves densitometry of complex mixtures of biopolymers resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis

Corrado Rizzi, Katia Rossini, Adonella Bruson, Marco Sandri, Angelo Dal Belin Peruffo, Ugo Carraro

Research output: Contribution to journalArticlepeer-review

Abstract

Due to its high sensitivity, silver staining is a widely popular method for the revelation of biopolymers separated by both native and denaturing electrophoresis. A step-by-step method for the destaining and restaining of overdeveloped/overloaded silver-stained bands is described that is applicable to both proteins and nucleic acids. The procedure significantly improves densitometric analysis of gels that have been silver stained with either commercial kits or solutions made in-house. The method permits reproducible densitometry of silver-stained gels and allows quantification of both main and minor components in complex mixture of molecules resolved on the same gel slab. All steps may be interrupted and are readily reversible, allowing for facile densitometric analyses and photographic recording under optimized conditions. Furthermore, common artifacts such as differential staining of the two gel surfaces, localized uneven yellow-ochre background, and the presence of fold marks and fingerprints can be easily removed.

Original languageEnglish
Pages (from-to)3266-3269
Number of pages4
JournalElectrophoresis
Volume23
Issue number19
DOIs
Publication statusPublished - Sep 2002

Keywords

  • Biopolymers
  • Densitometry
  • Silver staining
  • Sodium dodecyl sulfate-polyacrylamide gel electrophoresis

ASJC Scopus subject areas

  • Clinical Biochemistry

Fingerprint Dive into the research topics of 'Fully reversible procedure for silver staining improves densitometry of complex mixtures of biopolymers resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis'. Together they form a unique fingerprint.

Cite this