Fumarase tumor suppressor gene and MET oncogene cooperate in upholding transformation and tumorigenesis

Barbara Costa, Daniela Dettori, Annalisa Lorenzato, Chiara Bardella, Nadia Coltella, Cosimo Martino, Cristina Cammarata, Peter Carmeliet, Martina Olivero, Maria Flavia Di Renzo

Research output: Contribution to journalArticlepeer-review


Loss of the fumarate hydratase (FH) tumor suppressor gene results in the development of benign tumors that rarely, but regrettably, progress to very aggressive cancers. Using mouse embryo fibroblasts (MEFs) to model transformation, we found that fh knockdown results in increased expression of the met oncogene-encoded tyrosine kinase receptor through hypoxia-inducible factor (hif) stabilization. MET-increased expression was alone able to stabilize hif, thus establishing a feed forward loop that might enforce tumor progression. The fh-defective MEFs showed increased motility and protection from apoptosis. Motility, but not survival, relied on hif-1α and was greatly enhanced by MET ligand hepatocyte growth factor. Met cooperated with a weakly oncogenic ras in making MEFs transformed and tumorigenic, as shown by in vitro and in vivo assays. Loss of fh was not equally effective by itself but enhanced the transformed and tumorigenic phenotype induced by ras and MET. Consistently, the rescue of fumarase expression abrogated the motogenic and transformed phenotype of fh-defective MEFs. In conclusion, the data suggest that the progression of tumors where FH is lost might be boosted by activation of the MET oncogene, which is able to drive cell-autonomous tumor progression and is a strong candidate for targeted therapy.

Original languageEnglish
Pages (from-to)2680-2688
Number of pages9
JournalFASEB Journal
Issue number8
Publication statusPublished - Aug 2010


  • Hypoxia inducible factor
  • Mitochondrial tumor suppressors
  • Tyrosine kinase oncogenes

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Genetics
  • Molecular Biology


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