Function of glycoprotein Ibα in platelet activation induced by α-thrombin

L. De Marco, M. Mazzucato, A. Masotti, J. W. Fenton, Z. M. Ruggeri

Research output: Contribution to journalArticle

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Abstract

We have obtained evidence that selective inhibition of high affinity thrombin-binding sites located in the amino-terminal domain of the membrane glycoprotein (GP) Ibα results in impaired platelet activation, as shown by abrogation or reduction of the following responses induced in normal platelets by exposure to 2+](i)), (ii) release of dense granule content, (iii) binding of fibrinogen, (iv) aggregation. An anti-GP Ib monoclonal antibody, LJ-Ib10, which does not inhibit von Willebrand factor binding to platelets, obliterated the high affinity α-thrombin-binding sites on normal platelets. Isotherms of α-thrombin binding to normal platelets treated with saturating amounts of the antibody were virtually identical to those obtained with platelets from a patient with classical Bernard-Soulier syndrome. In parallel with decreased binding of the agonist, this antibody caused 50% inhibition of the maximal extent of platelet aggregation and 90% inhibition of ATP release induced by 0.3 nM α-thrombin. By inhibiting α-thrombin binding to GP Ib, the antibody prevented the activation of platelets exposed to low concentrations of the agonist, as demonstrated by abrogation of the increase in intraplatelet ionized calcium concentration induced in control platelets by 0.18 nM α-thrombin; under these conditions, fibrinogen binding was inhibited by 84%. Therefore, there is a correlation between occupancy of the high affinity sites for α-thrombin on GP Ibα and platelet activation, secretion, and aggregation, suggesting that GP Ibα is part of an α-thrombin receptor relevant for platelet function.

Original languageEnglish
Pages (from-to)23776-23783
Number of pages8
JournalJournal of Biological Chemistry
Volume266
Issue number35
Publication statusPublished - 1991

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Platelet Glycoprotein GPIb-IX Complex
Platelet Activation
Platelets
Thrombin
Blood Platelets
Chemical activation
Platelet Aggregation
Fibrinogen
Antibodies
Bernard-Soulier Syndrome
Agglomeration
Binding Sites
Thrombin Receptors
Membrane Glycoproteins
von Willebrand Factor
Adenosine Triphosphate
Monoclonal Antibodies
Calcium
Isotherms

ASJC Scopus subject areas

  • Biochemistry

Cite this

De Marco, L., Mazzucato, M., Masotti, A., Fenton, J. W., & Ruggeri, Z. M. (1991). Function of glycoprotein Ibα in platelet activation induced by α-thrombin. Journal of Biological Chemistry, 266(35), 23776-23783.

Function of glycoprotein Ibα in platelet activation induced by α-thrombin. / De Marco, L.; Mazzucato, M.; Masotti, A.; Fenton, J. W.; Ruggeri, Z. M.

In: Journal of Biological Chemistry, Vol. 266, No. 35, 1991, p. 23776-23783.

Research output: Contribution to journalArticle

De Marco, L, Mazzucato, M, Masotti, A, Fenton, JW & Ruggeri, ZM 1991, 'Function of glycoprotein Ibα in platelet activation induced by α-thrombin', Journal of Biological Chemistry, vol. 266, no. 35, pp. 23776-23783.
De Marco, L. ; Mazzucato, M. ; Masotti, A. ; Fenton, J. W. ; Ruggeri, Z. M. / Function of glycoprotein Ibα in platelet activation induced by α-thrombin. In: Journal of Biological Chemistry. 1991 ; Vol. 266, No. 35. pp. 23776-23783.
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