Functional analysis of a CDKN2A 5’UTR germline variant associated with pancreatic cancer development

William Bruno, Virginia Andreotti, Alessandra Bisio, Lorenza Pastorino, Giuseppe Fornarini, Stefania Sciallero, Giovanna Bianchi-Scarrà, Alberto Inga, Paola Ghiorzo

Research output: Contribution to journalArticle

Abstract

CDKN2A coding region germline variants are associated with pancreatic adenocarcinoma (PC) susceptibility. Recently, we described functional germline 5’UTR CDKN2A variants from melanoma patients affecting the post-transcriptional regulation of p16INK4a mRNA that is dependent, at least in part, on an Internal Ribosome Entry Site (IRES) in the 5’UTR region. Here we describe a 5’UTR c.-201_-198delinsCTTT CDKN2A variant (frequency 0.0028 based on 350 PC patients), which seems to be private to PC, since it has never been found in public databases nor in thousands of melanoma patients tested. Functional analyses confirmed IRES activity of the 5’UTR in BX-PC3 PC cells and revealed a functional impact of the identified variant. Using gene reporter assays we observed reduced translation potential in cells treated with the mTOR inhibitor Torin1, a condition that favors the assessment of IRES activity. At the endogenous gene level we quantified allelic imbalance among polysome-associated mRNAs using a patient-derived cell line heterozygous for the c.-201_-198delinsCTTT. Overall, we conclude that this very rare private variant can be considered a potential mutation, specifically associated with PC. Our data indicate that sequencing of the entire 5’UTR of CDKN2A should be included in routine screening of PC cases with suspected inherited susceptibility.

Original languageEnglish
Article numbere0189123
JournalPLoS One
Volume12
Issue number12
DOIs
Publication statusPublished - Dec 1 2017

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pancreatic neoplasms
Functional analysis
adenocarcinoma
Pancreatic Neoplasms
germ cells
Adenocarcinoma
Genes
Messenger RNA
ribosomes
Assays
Screening
Cells
melanoma
Melanoma
Allelic Imbalance
polyribosomes
Polyribosomes
Reporter Genes
reporter genes
translation (genetics)

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Functional analysis of a CDKN2A 5’UTR germline variant associated with pancreatic cancer development. / Bruno, William; Andreotti, Virginia; Bisio, Alessandra; Pastorino, Lorenza; Fornarini, Giuseppe; Sciallero, Stefania; Bianchi-Scarrà, Giovanna; Inga, Alberto; Ghiorzo, Paola.

In: PLoS One, Vol. 12, No. 12, e0189123, 01.12.2017.

Research output: Contribution to journalArticle

Bruno, William ; Andreotti, Virginia ; Bisio, Alessandra ; Pastorino, Lorenza ; Fornarini, Giuseppe ; Sciallero, Stefania ; Bianchi-Scarrà, Giovanna ; Inga, Alberto ; Ghiorzo, Paola. / Functional analysis of a CDKN2A 5’UTR germline variant associated with pancreatic cancer development. In: PLoS One. 2017 ; Vol. 12, No. 12.
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