Functional analysis of a murine monoclonal antibody against the repetitive region of the fibronectin-binding adhesins fibronectin-binding protein A and fibronectin-binding protein B from Staphylococcus aureus

Giulio Provenza, Maria Provenzano, Livia Visai, Fiona M. Burke, Joan A. Geoghegan, Matteo Stravalaci, Marco Gobbi, Giuliano Mazzini, Carla Renata Arciola, Timothy J. Foster, Pietro Speziale

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Fibronectin-binding proteins A and B are multifunctional LPXTG staphylococcal adhesins, comprising an N-terminal region that binds fibrinogen and elastin, and a C-terminal domain that interacts with fibronectin. The C-terminal domain of fibronectin-binding protein A is organized into 11 tandem repeats, six of which bind the ligand with high affinity; other sites bind more weakly. Fibronectin-binding protein B has been postulated to harbor 10 rather than 11 repeats, but it contains the same number of highaffinity fibronectin-binding sites as fibronectin-binding protein A. In this study, we confirm this prediction and show that six of 10 sites bind with dissociation constants in the nanomolar range. We also found that the fulllength repetitive region of fibronectin-binding protein B stimulated the production of a mAb (15E11) that binds with high affinity to an epitope shared by repeats 9 and 10 from both adhesins. With the use of truncated fragments of repeat 9 of fibronectin-binding protein A, we mapped the antibody epitope to the N-terminal segment and the fibronectin-binding site to the C-terminal segment of the repeat. The distinct localization of the 15E11 epitope and the fibronectin-binding site suggests that the interfering effect of the antibody might result from steric hindrance or a conformational change in the structure that reduces the accessibility of fibronectin to its binding determinant. The epitope is well exposed on the surface of staphylococcal cells, as determined by genetic analyses, fluorescence microscopy, and flow cytometry. When incubated with cells of Staphylococcs aureus strains, 15E11 inhibits attachment of bacteria to surface-coated fibronectin by almost 70%.

Original languageEnglish
Pages (from-to)4490-4505
Number of pages16
JournalFEBS Journal
Volume277
Issue number21
DOIs
Publication statusPublished - Nov 2010

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Functional analysis
Nucleic Acid Repetitive Sequences
Fibronectins
Staphylococcus aureus
Monoclonal Antibodies
Epitopes
Binding Sites
Tandem Repeat Sequences
Flow cytometry
Elastin
Antibodies
Fluorescence microscopy
Ports and harbors
Terminal Repeat Sequences
Fibrinogen
bacterial fibronectin-binding proteins
Fluorescence Microscopy
Bacteria
Flow Cytometry
Ligands

Keywords

  • Adhesin
  • Antibody
  • Fibronectin
  • Repeat
  • Staphylococcus

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology
  • Medicine(all)

Cite this

Functional analysis of a murine monoclonal antibody against the repetitive region of the fibronectin-binding adhesins fibronectin-binding protein A and fibronectin-binding protein B from Staphylococcus aureus. / Provenza, Giulio; Provenzano, Maria; Visai, Livia; Burke, Fiona M.; Geoghegan, Joan A.; Stravalaci, Matteo; Gobbi, Marco; Mazzini, Giuliano; Arciola, Carla Renata; Foster, Timothy J.; Speziale, Pietro.

In: FEBS Journal, Vol. 277, No. 21, 11.2010, p. 4490-4505.

Research output: Contribution to journalArticle

Provenza, Giulio ; Provenzano, Maria ; Visai, Livia ; Burke, Fiona M. ; Geoghegan, Joan A. ; Stravalaci, Matteo ; Gobbi, Marco ; Mazzini, Giuliano ; Arciola, Carla Renata ; Foster, Timothy J. ; Speziale, Pietro. / Functional analysis of a murine monoclonal antibody against the repetitive region of the fibronectin-binding adhesins fibronectin-binding protein A and fibronectin-binding protein B from Staphylococcus aureus. In: FEBS Journal. 2010 ; Vol. 277, No. 21. pp. 4490-4505.
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AU - Stravalaci, Matteo

AU - Gobbi, Marco

AU - Mazzini, Giuliano

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