Functional analysis of pRb2/p130 interaction with cyclins

Pier Paolo Claudio, Antonio De Luca, Candace M. Howard, Alfonso Baldi, Eduardo J. Firpo, Andrew Koff, Marco G. Paggi, Antonio Giordano

Research output: Contribution to journalArticlepeer-review

Abstract

The retinoblastoma (Rb) family consists of the tumor suppressor pRb and related proteins p107 and pRb2/p130. Ectopic expression of pRb and p107 results in a growth arrest of sensitive cells in the G1 phase of the cell cycle. We demonstrated here that the growth-suppressive properties of pRb2/p130 were also specific for the G1 phase. The A-, E-, and D-type cyclins as well as transcription factor E2F1 and the E1A viral oncoprotein were able to rescue the pRb2/p130-mediated G1 growth arrest in SAOS-2 cells. The rescue with cyclins A and E correlated with their physical interaction with pRb2/p130, which surprisingly has been found to occur over all phases of the cell cycle. The phosphorylation status as well as the kinase activity associated with pRb2/p130 dramatically increased near the G1-S-phase transition. This suggests that, like the other Rb family members, pRb and p107, the phosphorylation of pRb2/p130 is controlled by the cell cycle machinery and that pRb2/p130 may indeed be another key G1-S-phase regulator.

Original languageEnglish
Pages (from-to)2003-2008
Number of pages6
JournalCancer Research
Volume56
Issue number9
Publication statusPublished - May 1 1996

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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