Functional analysis of T lymphocytes infiltrating the dermis and epidermis of post-burn hypertrophic scar tissues

Paola Bernabei, Laura Rigamonti, Silvia Ariotti, Maurizio Stella, Carlotta Castagnoli, Francesco Novelli

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The cytokine profile of T cell clones (TCC) from the dermis and epidermis of burn patients with hypertrophic scars (HS) in active (AHS) and remission phases (RHS) was determined in this study. We found that AHS tissues are heavily infiltrated by Type 0-Type 1 polarized CD3+ lymphocytes producing high IFN-γ and low IL-4 levels. Analysis of their surface marker phenotype showed that the high IFN-γ production was shared equally between the CD4+ TCRα/β and CD8+ TCRα/β clones. The profile of TCC from RHS tissues revealed pronounced infiltration of Type 0-Type 1 polarized lymphocytes with an even more evident Type 1 profile. However, the levels of IFN-γ produced by RHS-derived TCC were 4-6 times lower than those produced by AHS-derived TCC. These data show that high levels of IFN-γ produced by Type 0-Type 1 lymphocytes infiltrating HS are a feature of AHS, whereas reduction of this ability to produce high levels of IFN-γ, though without a shift towards a Type 0-Type 2 phenotype through an increase in IL-4, is characteristic of RHS.

Original languageEnglish
Pages (from-to)43-48
Number of pages6
JournalBurns
Volume25
Issue number1
DOIs
Publication statusPublished - Feb 1999

Fingerprint

Hypertrophic Cicatrix
Dermis
Burns
Epidermis
Clone Cells
T-Lymphocytes
Lymphocytes
Interleukin-4
Phenotype
Cytokines

Keywords

  • Burns
  • Cytokines
  • Hypertrophic scars
  • Th1/Th2

ASJC Scopus subject areas

  • Emergency Medicine
  • Surgery

Cite this

Functional analysis of T lymphocytes infiltrating the dermis and epidermis of post-burn hypertrophic scar tissues. / Bernabei, Paola; Rigamonti, Laura; Ariotti, Silvia; Stella, Maurizio; Castagnoli, Carlotta; Novelli, Francesco.

In: Burns, Vol. 25, No. 1, 02.1999, p. 43-48.

Research output: Contribution to journalArticle

Bernabei, Paola ; Rigamonti, Laura ; Ariotti, Silvia ; Stella, Maurizio ; Castagnoli, Carlotta ; Novelli, Francesco. / Functional analysis of T lymphocytes infiltrating the dermis and epidermis of post-burn hypertrophic scar tissues. In: Burns. 1999 ; Vol. 25, No. 1. pp. 43-48.
@article{584aed53bf3d455a859fe47addebf320,
title = "Functional analysis of T lymphocytes infiltrating the dermis and epidermis of post-burn hypertrophic scar tissues",
abstract = "The cytokine profile of T cell clones (TCC) from the dermis and epidermis of burn patients with hypertrophic scars (HS) in active (AHS) and remission phases (RHS) was determined in this study. We found that AHS tissues are heavily infiltrated by Type 0-Type 1 polarized CD3+ lymphocytes producing high IFN-γ and low IL-4 levels. Analysis of their surface marker phenotype showed that the high IFN-γ production was shared equally between the CD4+ TCRα/β and CD8+ TCRα/β clones. The profile of TCC from RHS tissues revealed pronounced infiltration of Type 0-Type 1 polarized lymphocytes with an even more evident Type 1 profile. However, the levels of IFN-γ produced by RHS-derived TCC were 4-6 times lower than those produced by AHS-derived TCC. These data show that high levels of IFN-γ produced by Type 0-Type 1 lymphocytes infiltrating HS are a feature of AHS, whereas reduction of this ability to produce high levels of IFN-γ, though without a shift towards a Type 0-Type 2 phenotype through an increase in IL-4, is characteristic of RHS.",
keywords = "Burns, Cytokines, Hypertrophic scars, Th1/Th2",
author = "Paola Bernabei and Laura Rigamonti and Silvia Ariotti and Maurizio Stella and Carlotta Castagnoli and Francesco Novelli",
year = "1999",
month = "2",
doi = "10.1016/S0305-4179(98)00128-4",
language = "English",
volume = "25",
pages = "43--48",
journal = "Burns",
issn = "0305-4179",
publisher = "Elsevier Limited",
number = "1",

}

TY - JOUR

T1 - Functional analysis of T lymphocytes infiltrating the dermis and epidermis of post-burn hypertrophic scar tissues

AU - Bernabei, Paola

AU - Rigamonti, Laura

AU - Ariotti, Silvia

AU - Stella, Maurizio

AU - Castagnoli, Carlotta

AU - Novelli, Francesco

PY - 1999/2

Y1 - 1999/2

N2 - The cytokine profile of T cell clones (TCC) from the dermis and epidermis of burn patients with hypertrophic scars (HS) in active (AHS) and remission phases (RHS) was determined in this study. We found that AHS tissues are heavily infiltrated by Type 0-Type 1 polarized CD3+ lymphocytes producing high IFN-γ and low IL-4 levels. Analysis of their surface marker phenotype showed that the high IFN-γ production was shared equally between the CD4+ TCRα/β and CD8+ TCRα/β clones. The profile of TCC from RHS tissues revealed pronounced infiltration of Type 0-Type 1 polarized lymphocytes with an even more evident Type 1 profile. However, the levels of IFN-γ produced by RHS-derived TCC were 4-6 times lower than those produced by AHS-derived TCC. These data show that high levels of IFN-γ produced by Type 0-Type 1 lymphocytes infiltrating HS are a feature of AHS, whereas reduction of this ability to produce high levels of IFN-γ, though without a shift towards a Type 0-Type 2 phenotype through an increase in IL-4, is characteristic of RHS.

AB - The cytokine profile of T cell clones (TCC) from the dermis and epidermis of burn patients with hypertrophic scars (HS) in active (AHS) and remission phases (RHS) was determined in this study. We found that AHS tissues are heavily infiltrated by Type 0-Type 1 polarized CD3+ lymphocytes producing high IFN-γ and low IL-4 levels. Analysis of their surface marker phenotype showed that the high IFN-γ production was shared equally between the CD4+ TCRα/β and CD8+ TCRα/β clones. The profile of TCC from RHS tissues revealed pronounced infiltration of Type 0-Type 1 polarized lymphocytes with an even more evident Type 1 profile. However, the levels of IFN-γ produced by RHS-derived TCC were 4-6 times lower than those produced by AHS-derived TCC. These data show that high levels of IFN-γ produced by Type 0-Type 1 lymphocytes infiltrating HS are a feature of AHS, whereas reduction of this ability to produce high levels of IFN-γ, though without a shift towards a Type 0-Type 2 phenotype through an increase in IL-4, is characteristic of RHS.

KW - Burns

KW - Cytokines

KW - Hypertrophic scars

KW - Th1/Th2

UR - http://www.scopus.com/inward/record.url?scp=0033083595&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033083595&partnerID=8YFLogxK

U2 - 10.1016/S0305-4179(98)00128-4

DO - 10.1016/S0305-4179(98)00128-4

M3 - Article

VL - 25

SP - 43

EP - 48

JO - Burns

JF - Burns

SN - 0305-4179

IS - 1

ER -