Functional behaviour and immunological phenotype of circulating B lymphocytes in multiple myeloma. Studies with pokeweed mitogen

M. Gobbi, F. Caligaris-Cappio, D. Campana, P. L. Tazzari, L. Bergui, M. Cavo, S. Tura

Research output: Contribution to journalArticlepeer-review

Abstract

Peripheral blood B lymphocytes, depleted of adherent cells, from 10 patients with multiple myeloma were cultured in the presence of PWM with autologous or donor T lymphocytes. The results show that: (1) co-cultures with allogeneic T lymphocytes produced more plasma cells than those with autologous ones; (2) the κ/λ ratio overlapped the values obtained in normal controls, irrespective of the light chain produced by the neoplastic plasma cells and (3) the immunological phenotype of plasma cells obtained from PWM stimulated peripheral B cells (RFA2 +, RFA3 +, A10 +) was clearly different from that one of myelomatous plasma cells (RFA2 -, RFA3 -, A10 +). These data confirm the T cell imbalance already seen in myeloma patients; moreover they show that PWM responsive B cells are functionally normal and phenotypically different from bone marrow myeloma cells. These results support the view that most of the peripheral B lymphocytes, previously identified as monoclonal are in fact normal cells bearing adherent monoclonal Ig molecules.

Original languageEnglish
Pages (from-to)625-630
Number of pages6
JournalClinical and Experimental Immunology
Volume58
Issue number3
Publication statusPublished - 1984

ASJC Scopus subject areas

  • Immunology

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