Functional characterization of a human DNase-like protein encoded by a gene positioned in Xq28

Valentina Appierto, Lidia Bardella, C. Vijayasarathy, Narayan Avadhani, Vittorio Sgaramella, Ida Biunno

Research output: Contribution to journalArticle

Abstract

Xib, a gene recently reported to reside on the q28 region of the human X chromosome, contains an open reading frame homologous to those of the DNase I family enzymes. The full open reading frame of this gene has been fused to the E. coli gene of the maltose binding protein and expressed in bacteria as a chimeric protein. The partially purified chimeric protein is enzymatically active. It introduces single and double stranded breaks into supercoiled DNA, at 30°C in the absence of divalent cations and at a pH optimum of 5.2. To our knowledge this enzyme represents the first cloned human endonuclease with characteristics similar to those of acidic DNase II.

Original languageEnglish
Pages (from-to)119-122
Number of pages4
JournalGene
Volume188
Issue number1
DOIs
Publication statusPublished - Mar 25 1997

Keywords

  • Acidic DNase
  • Endonuclease
  • Human chromosome X

ASJC Scopus subject areas

  • Genetics

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    Appierto, V., Bardella, L., Vijayasarathy, C., Avadhani, N., Sgaramella, V., & Biunno, I. (1997). Functional characterization of a human DNase-like protein encoded by a gene positioned in Xq28. Gene, 188(1), 119-122. https://doi.org/10.1016/S0378-1119(96)00794-9