Functional characterization of cloned intrahepatic, hepatitis B virus nucleoprotein-specific helper T cell lines

C. Ferrari, M. U. Mondelli, A. Penna, F. Fiaccadori, F. V. Chisari

Research output: Contribution to journalArticle

Abstract

We have previously reported the establishment and preliminary characterization of polyclonal hepatitis B virus (HBV) nucleoprotein (HBcAg)-specific CD4+ and CD8+ T cell lines derived from the hepatic lymphomononuclear cell infiltrate of several patients with chronic active hepatitis B. The isolated subsets from these lines were specifically activated by HBcAg and displayed antigen-specific help and suppression with respect to proliferation of the alternate subset. One of these lines were recently cloned by limiting dilution, and four HBcAg-specific CD3+ CD4+ CD8- DR+ T cell lines were produced that had a 95.3% likelihood of monoclonality. Antigenic specificity was confirmed by dose-dependent, HLA class II (DR)-restricted proliferation in response to recombinant and human serum-derived HBcAg and the lack of proliferation to HBV envelope antigens (HBsAg and pre-S(2)Ag). All cloned lines were interleukin 2 dependent, produced interferon-γ in an antigen-specific manner, and provided antigen-specific help to autologous B cells with respect to anti-HBc production. We conclude that HBcAg-specific, HLA-class II restricted helper T cells capable of inducing antigen-specific functional responses by autologous B lymphocytes and T lymphocytes are present at the site of viral antigen synthesis and hepatocellular injury in HBV infection.

Original languageEnglish
Pages (from-to)539-544
Number of pages6
JournalJournal of Immunology
Volume139
Issue number2
Publication statusPublished - 1987

ASJC Scopus subject areas

  • Immunology

Fingerprint Dive into the research topics of 'Functional characterization of cloned intrahepatic, hepatitis B virus nucleoprotein-specific helper T cell lines'. Together they form a unique fingerprint.

  • Cite this