Abstract
The CG18317 gene (drim2) is the Drosophila melanogaster homolog of the Saccharomyces cerevisiae Rim2 gene, which encodes a pyrimidine (deoxy)nucleotide carrier. Here, we tested if the drim2 gene also encodes for a deoxynucleotide transporter in the fruit fly. The protein was localized to mitochondria. Drosophila S2R+ cells, silenced for drim2 expression, contained markedly reduced pools of both purine and pyrimidine dNTPs in mitochondria, whereas cytosolic pools were unaffected. In vivo drim2 homozygous knock-out was lethal at the larval stage, preceded by the following: (i) impaired locomotor behavior; (ii) decreased rates of oxygen consumption, and (iii) depletion of mtDNA. We conclude that the Drosophila mitochondrial carrier dRIM2 transports all DNA precursors and is essential to maintain mitochondrial function.
| Original language | English |
|---|---|
| Pages (from-to) | 7448-7459 |
| Number of pages | 12 |
| Journal | Journal of Biological Chemistry |
| Volume | 289 |
| Issue number | 11 |
| DOIs | |
| Publication status | Published - Mar 14 2014 |
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ASJC Scopus subject areas
- Biochemistry
- Cell Biology
- Molecular Biology
Cite this
Functional characterization of drim2, the Drosophila melanogaster homolog of the yeast mitochondrial deoxynucleotide transporter. / Da-Rè, Caterina; Franzolin, Elisa; Biscontin, Alberto; Piazzesi, Antonia; Pacchioni, Beniamina; Gagliani, Maria Cristina; Mazzotta, Gabriella; Tacchetti, Carlo; Zordan, Mauro A.; Zeviani, Massimo; Bernardi, Paolo; Bianchi, Vera; De Pittà, Cristiano; Costa, Rodolfo.
In: Journal of Biological Chemistry, Vol. 289, No. 11, 14.03.2014, p. 7448-7459.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Functional characterization of drim2, the Drosophila melanogaster homolog of the yeast mitochondrial deoxynucleotide transporter
AU - Da-Rè, Caterina
AU - Franzolin, Elisa
AU - Biscontin, Alberto
AU - Piazzesi, Antonia
AU - Pacchioni, Beniamina
AU - Gagliani, Maria Cristina
AU - Mazzotta, Gabriella
AU - Tacchetti, Carlo
AU - Zordan, Mauro A.
AU - Zeviani, Massimo
AU - Bernardi, Paolo
AU - Bianchi, Vera
AU - De Pittà, Cristiano
AU - Costa, Rodolfo
PY - 2014/3/14
Y1 - 2014/3/14
N2 - The CG18317 gene (drim2) is the Drosophila melanogaster homolog of the Saccharomyces cerevisiae Rim2 gene, which encodes a pyrimidine (deoxy)nucleotide carrier. Here, we tested if the drim2 gene also encodes for a deoxynucleotide transporter in the fruit fly. The protein was localized to mitochondria. Drosophila S2R+ cells, silenced for drim2 expression, contained markedly reduced pools of both purine and pyrimidine dNTPs in mitochondria, whereas cytosolic pools were unaffected. In vivo drim2 homozygous knock-out was lethal at the larval stage, preceded by the following: (i) impaired locomotor behavior; (ii) decreased rates of oxygen consumption, and (iii) depletion of mtDNA. We conclude that the Drosophila mitochondrial carrier dRIM2 transports all DNA precursors and is essential to maintain mitochondrial function.
AB - The CG18317 gene (drim2) is the Drosophila melanogaster homolog of the Saccharomyces cerevisiae Rim2 gene, which encodes a pyrimidine (deoxy)nucleotide carrier. Here, we tested if the drim2 gene also encodes for a deoxynucleotide transporter in the fruit fly. The protein was localized to mitochondria. Drosophila S2R+ cells, silenced for drim2 expression, contained markedly reduced pools of both purine and pyrimidine dNTPs in mitochondria, whereas cytosolic pools were unaffected. In vivo drim2 homozygous knock-out was lethal at the larval stage, preceded by the following: (i) impaired locomotor behavior; (ii) decreased rates of oxygen consumption, and (iii) depletion of mtDNA. We conclude that the Drosophila mitochondrial carrier dRIM2 transports all DNA precursors and is essential to maintain mitochondrial function.
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UR - http://www.scopus.com/inward/citedby.url?scp=84896308907&partnerID=8YFLogxK
U2 - 10.1074/jbc.M113.543926
DO - 10.1074/jbc.M113.543926
M3 - Article
C2 - 24469456
AN - SCOPUS:84896308907
VL - 289
SP - 7448
EP - 7459
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 11
ER -