Functional characterization of drim2, the Drosophila melanogaster homolog of the yeast mitochondrial deoxynucleotide transporter

Caterina Da-Rè, Elisa Franzolin, Alberto Biscontin, Antonia Piazzesi, Beniamina Pacchioni, Maria Cristina Gagliani, Gabriella Mazzotta, Carlo Tacchetti, Mauro A. Zordan, Massimo Zeviani, Paolo Bernardi, Vera Bianchi, Cristiano De Pittà, Rodolfo Costa

Research output: Contribution to journalArticlepeer-review

Abstract

The CG18317 gene (drim2) is the Drosophila melanogaster homolog of the Saccharomyces cerevisiae Rim2 gene, which encodes a pyrimidine (deoxy)nucleotide carrier. Here, we tested if the drim2 gene also encodes for a deoxynucleotide transporter in the fruit fly. The protein was localized to mitochondria. Drosophila S2R+ cells, silenced for drim2 expression, contained markedly reduced pools of both purine and pyrimidine dNTPs in mitochondria, whereas cytosolic pools were unaffected. In vivo drim2 homozygous knock-out was lethal at the larval stage, preceded by the following: (i) impaired locomotor behavior; (ii) decreased rates of oxygen consumption, and (iii) depletion of mtDNA. We conclude that the Drosophila mitochondrial carrier dRIM2 transports all DNA precursors and is essential to maintain mitochondrial function.

Original languageEnglish
Pages (from-to)7448-7459
Number of pages12
JournalJournal of Biological Chemistry
Volume289
Issue number11
DOIs
Publication statusPublished - Mar 14 2014

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

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