Functional characterization of drim2, the Drosophila melanogaster homolog of the yeast mitochondrial deoxynucleotide transporter

Caterina Da-Rè, Elisa Franzolin, Alberto Biscontin, Antonia Piazzesi, Beniamina Pacchioni, Maria Cristina Gagliani, Gabriella Mazzotta, Carlo Tacchetti, Mauro A. Zordan, Massimo Zeviani, Paolo Bernardi, Vera Bianchi, Cristiano De Pittà, Rodolfo Costa

Research output: Contribution to journalArticle

Abstract

The CG18317 gene (drim2) is the Drosophila melanogaster homolog of the Saccharomyces cerevisiae Rim2 gene, which encodes a pyrimidine (deoxy)nucleotide carrier. Here, we tested if the drim2 gene also encodes for a deoxynucleotide transporter in the fruit fly. The protein was localized to mitochondria. Drosophila S2R+ cells, silenced for drim2 expression, contained markedly reduced pools of both purine and pyrimidine dNTPs in mitochondria, whereas cytosolic pools were unaffected. In vivo drim2 homozygous knock-out was lethal at the larval stage, preceded by the following: (i) impaired locomotor behavior; (ii) decreased rates of oxygen consumption, and (iii) depletion of mtDNA. We conclude that the Drosophila mitochondrial carrier dRIM2 transports all DNA precursors and is essential to maintain mitochondrial function.

Original languageEnglish
Pages (from-to)7448-7459
Number of pages12
JournalJournal of Biological Chemistry
Volume289
Issue number11
DOIs
Publication statusPublished - Mar 14 2014

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Drosophila melanogaster
Yeast
Mitochondria
Genes
Yeasts
Drosophila
Pyrimidine Nucleotides
Carrier transport
Fruits
Mitochondrial DNA
Oxygen Consumption
Diptera
Saccharomyces cerevisiae
Fruit
Nucleotides
Oxygen
DNA
Proteins
pyrimidine

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Da-Rè, C., Franzolin, E., Biscontin, A., Piazzesi, A., Pacchioni, B., Gagliani, M. C., ... Costa, R. (2014). Functional characterization of drim2, the Drosophila melanogaster homolog of the yeast mitochondrial deoxynucleotide transporter. Journal of Biological Chemistry, 289(11), 7448-7459. https://doi.org/10.1074/jbc.M113.543926

Functional characterization of drim2, the Drosophila melanogaster homolog of the yeast mitochondrial deoxynucleotide transporter. / Da-Rè, Caterina; Franzolin, Elisa; Biscontin, Alberto; Piazzesi, Antonia; Pacchioni, Beniamina; Gagliani, Maria Cristina; Mazzotta, Gabriella; Tacchetti, Carlo; Zordan, Mauro A.; Zeviani, Massimo; Bernardi, Paolo; Bianchi, Vera; De Pittà, Cristiano; Costa, Rodolfo.

In: Journal of Biological Chemistry, Vol. 289, No. 11, 14.03.2014, p. 7448-7459.

Research output: Contribution to journalArticle

Da-Rè, C, Franzolin, E, Biscontin, A, Piazzesi, A, Pacchioni, B, Gagliani, MC, Mazzotta, G, Tacchetti, C, Zordan, MA, Zeviani, M, Bernardi, P, Bianchi, V, De Pittà, C & Costa, R 2014, 'Functional characterization of drim2, the Drosophila melanogaster homolog of the yeast mitochondrial deoxynucleotide transporter', Journal of Biological Chemistry, vol. 289, no. 11, pp. 7448-7459. https://doi.org/10.1074/jbc.M113.543926
Da-Rè, Caterina ; Franzolin, Elisa ; Biscontin, Alberto ; Piazzesi, Antonia ; Pacchioni, Beniamina ; Gagliani, Maria Cristina ; Mazzotta, Gabriella ; Tacchetti, Carlo ; Zordan, Mauro A. ; Zeviani, Massimo ; Bernardi, Paolo ; Bianchi, Vera ; De Pittà, Cristiano ; Costa, Rodolfo. / Functional characterization of drim2, the Drosophila melanogaster homolog of the yeast mitochondrial deoxynucleotide transporter. In: Journal of Biological Chemistry. 2014 ; Vol. 289, No. 11. pp. 7448-7459.
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