Functional characterization of natural killer cells in type I leukocyte adhesion deficiency

Roberta Castriconi, Alessandra Dondero, Claudia Cantoni, Mariella Della Chiesa, Carola Prato, Marina Nanni, Maurilia Fiorini, Lucia Notarangelo, Silvia Parolini, Lorenzo Moretta, Luigi Notarangelo, Alessandro Moretta, Cristina Bottino

Research output: Contribution to journalArticlepeer-review

Abstract

In this study, we analyzed IL-2-activated polyclonal natural killer (NK) cells derived from 2 patients affected by leukocyte adhesion deficiency type I (LAD1), an immunodeficiency characterized by mutations of the gene coding for CD18, the β subunit shared by major leukocyte integrins. We show that LAD1 NK cells express normal levels of various triggering NK receptors (and coreceptors) and that mAb-mediated engagement of these receptors results in the enhancement of both NK cytolytic activity and cytokine production. Moreover, these activating NK receptors were capable of recognizing their specific ligands on target cells. Thus, LAD1 NK cells, similarly to normal NK cells, were capable of killing most human tumor cells analyzed and produced high amounts of IFN-γ when cocultured in presence of target cells. Murine target cells represented a common exception, as they were poorly susceptible to LAD1 NK cells. Finally, LAD1 NK cells could efficiently kill or induce maturation of monocyte-derived immature dendritic cells (DCs). Altogether our present study indicates that in LAD1 patients, 3 important functions of NK cells (eg, cytotoxicity, IFN-γ production, and DC editing) are only marginally affected and provides new insight on the cooperation between activating receptors and LFA-1 in the induction of NK cell activation and function.

Original languageEnglish
Pages (from-to)4873-4881
Number of pages9
JournalBlood
Volume109
Issue number11
DOIs
Publication statusPublished - Jun 1 2007

ASJC Scopus subject areas

  • Hematology

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