Functional HIV-1 specific IgA antibodies in HIV-1 exposed, persistently IgG seronegative female sex workers

Kristina Broliden, Jorma Hinkula, Claudia Devito, Peter Kiama, Joshua Kimani, Daria Trabbatoni, Job J. Bwayo, Mario Clerici, Francis Plummer, Rupert Kaul

Research output: Contribution to journalArticle

Abstract

Although HIV-specific cellular immune responses are found in a number of HIV highly-exposed, persistently seronegative (HEPS) cohorts, late seroconversion can occur despite pre-existing cytotoxic T lymphocytes (CTL), suggesting that a protective HIV vaccine may need to induce a broader range of HIV-specific immune responses. Low levels of HIV-specific IgA have been found in the genital tract and plasma of the majority of Nairobi HEPS sex workers and appeared to be independent of HIV-specific cellular responses. IgA purified from genital tract, saliva and plasma of most HEPS sex workers were able to neutralize infection of PBMC by a primary (NSI) clade B HIV isolate, as well as viral isolates from clades A and D, which predominate in Kenya. In addition, these IgA were able to inhibit transcytosis of infective HIV virions across a transwell model of the human mucosal epithelium in an HIV-specific manner. Preliminary work in other HEPS cohorts has suggested the recognition of different gp41 epitopes in HEPS and HIV-infected subjects. Although present at low levels, these IgA demonstrated cross-clade neutralizing activity and were able to inhibit HIV mucosal transcytosis, suggesting an important functional role in protection against HIV infection.

Original languageEnglish
Pages (from-to)29-36
Number of pages8
JournalImmunology Letters
Volume79
Issue number1-2
DOIs
Publication statusPublished - Nov 1 2001

Fingerprint

Sex Workers
Immunoglobulin A
HIV-1
Immunoglobulin G
HIV
Antibodies
Transcytosis
AIDS Vaccines
Kenya
Cytotoxic T-Lymphocytes
Saliva
Cellular Immunity
Virion
HIV Infections
Epitopes
Epithelium

Keywords

  • HIV resistance
  • Humoral immunity
  • Neutralization
  • Transcytosis

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Broliden, K., Hinkula, J., Devito, C., Kiama, P., Kimani, J., Trabbatoni, D., ... Kaul, R. (2001). Functional HIV-1 specific IgA antibodies in HIV-1 exposed, persistently IgG seronegative female sex workers. Immunology Letters, 79(1-2), 29-36. https://doi.org/10.1016/S0165-2478(01)00263-2

Functional HIV-1 specific IgA antibodies in HIV-1 exposed, persistently IgG seronegative female sex workers. / Broliden, Kristina; Hinkula, Jorma; Devito, Claudia; Kiama, Peter; Kimani, Joshua; Trabbatoni, Daria; Bwayo, Job J.; Clerici, Mario; Plummer, Francis; Kaul, Rupert.

In: Immunology Letters, Vol. 79, No. 1-2, 01.11.2001, p. 29-36.

Research output: Contribution to journalArticle

Broliden, K, Hinkula, J, Devito, C, Kiama, P, Kimani, J, Trabbatoni, D, Bwayo, JJ, Clerici, M, Plummer, F & Kaul, R 2001, 'Functional HIV-1 specific IgA antibodies in HIV-1 exposed, persistently IgG seronegative female sex workers', Immunology Letters, vol. 79, no. 1-2, pp. 29-36. https://doi.org/10.1016/S0165-2478(01)00263-2
Broliden, Kristina ; Hinkula, Jorma ; Devito, Claudia ; Kiama, Peter ; Kimani, Joshua ; Trabbatoni, Daria ; Bwayo, Job J. ; Clerici, Mario ; Plummer, Francis ; Kaul, Rupert. / Functional HIV-1 specific IgA antibodies in HIV-1 exposed, persistently IgG seronegative female sex workers. In: Immunology Letters. 2001 ; Vol. 79, No. 1-2. pp. 29-36.
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