TY - JOUR
T1 - Functional implications of microRNAs in crohn’s disease revealed by integrating microRNA and messenger RNA expression profiling
AU - Palmieri, Orazio
AU - Creanza, Teresa Maria
AU - Bossa, Fabrizio
AU - Latiano, Tiziana
AU - Corritore, Giuseppe
AU - Palumbo, Orazio
AU - Martino, Giuseppina
AU - Biscaglia, Giuseppe
AU - Scimeca, Daniela
AU - Carella, Massimo
AU - Ancona, Nicola
AU - Andriulli, Angelo
AU - Latiano, Anna
PY - 2017/7/20
Y1 - 2017/7/20
N2 - Crohn’s disease (CD) is a debilitating inflammatory bowel disease (IBD) that emerges due to the influence of genetic and environmental factors. microRNAs (miRNAs) have been identified in the tissue and sera of IBD patients and may play an important role in the induction of IBD. Our study aimed to identify differentially expressed miRNAs and miRNAs with the ability to alter transcriptome activity by comparing inflamed tissue samples with their non-inflamed counterparts. We studied changes in miRNA–mRNA interactions associated with CD by examining their differential co-expression relative to normal mucosa from the same patients. Correlation changes between the two conditions were incorporated into scores of predefined gene sets to identify biological processes with altered miRNA-mediated control. Our study identified 28 miRNAs differentially expressed (p-values < 0.01), of which 14 are up-regulated. Notably, our differential coexpression analysis highlights microRNAs (i.e., miR-4284, miR-3194 and miR-21) that have known functional interactions with key mechanisms implicated in IBD. Most of these miRNAs cannot be detected by differential expression analysis that do not take into account miRNA–mRNA interactions. The identification of differential miRNA–mRNA co-expression patterns will facilitate the investigation of the miRNA-mediated molecular mechanisms underlying CD pathogenesis and could suggest novel drug targets for validation.
AB - Crohn’s disease (CD) is a debilitating inflammatory bowel disease (IBD) that emerges due to the influence of genetic and environmental factors. microRNAs (miRNAs) have been identified in the tissue and sera of IBD patients and may play an important role in the induction of IBD. Our study aimed to identify differentially expressed miRNAs and miRNAs with the ability to alter transcriptome activity by comparing inflamed tissue samples with their non-inflamed counterparts. We studied changes in miRNA–mRNA interactions associated with CD by examining their differential co-expression relative to normal mucosa from the same patients. Correlation changes between the two conditions were incorporated into scores of predefined gene sets to identify biological processes with altered miRNA-mediated control. Our study identified 28 miRNAs differentially expressed (p-values < 0.01), of which 14 are up-regulated. Notably, our differential coexpression analysis highlights microRNAs (i.e., miR-4284, miR-3194 and miR-21) that have known functional interactions with key mechanisms implicated in IBD. Most of these miRNAs cannot be detected by differential expression analysis that do not take into account miRNA–mRNA interactions. The identification of differential miRNA–mRNA co-expression patterns will facilitate the investigation of the miRNA-mediated molecular mechanisms underlying CD pathogenesis and could suggest novel drug targets for validation.
KW - Crohn’s disease
KW - Differential expression analysis
KW - MicroRNA
KW - MicroRNA– mRNA co-expression
KW - mRNA
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UR - http://www.scopus.com/inward/citedby.url?scp=85025806249&partnerID=8YFLogxK
U2 - 10.3390/ijms18071580
DO - 10.3390/ijms18071580
M3 - Article
AN - SCOPUS:85025806249
VL - 18
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
SN - 1661-6596
IS - 7
M1 - 1580
ER -