Functional interaction of the subunit 3 of RNA polymerase II (RPB3) with transcription factor-4 (ATF4)

Roberta De Angelis, Simona Iezzi, Tiziana Bruno, Nicoletta Corbi, Monica Di Padova, Aristide Floridi, Maurizio Fanciulli, Claudio Passananti

Research output: Contribution to journalArticle

Abstract

RPB3 is a core subunit of RNA polymerase II (pol II) that, together with the RPB11 subunit, forms the heterodimer considered as a functional counterpart of the bacterial α subunit homodimer involved in promoter recognition. We previously employed the yeast two-hybrid system and identified an interaction between RPB3 and the myogenic transcription factor myogenin, demonstrating an involvement of this subunit in muscle differentiation. In this paper we report the interaction between RPB3 and another known transcription factor, ATF4. We found that the intensity of the interaction between RPB3 and ATF4 is similar to the one between RPB3 and myogenin. This interaction involves an RPB3 specific region not homologous to the prokaryotic α subunit. We demonstrated that RBP3 is able to enhance ATF4 transactivation, whereas the region of RPB3 (Sud) that contacts ATF4, when used as a dominant negative, markedly inhibits ATF4 transactivation activity. Interestingly, ATF4 protein level, as reported for its partner RPB3, increases during C2C7 cell line muscle differentiation.

Original languageEnglish
Pages (from-to)15-19
Number of pages5
JournalFEBS Letters
Volume547
Issue number1-3
DOIs
Publication statusPublished - Jul 17 2003

Keywords

  • ATF4
  • Muscle differentiation
  • RNA polymerase II
  • RPB3

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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