Functional modulation by lactate of myoglobin. A monomeric allosteric hemoprotein

Bruno Giardina, Paolo Ascenzi, Maria Elisabetta Clementi, Giampiero De Sanctis, Menico Rizzi, Massimo Coletta

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

The effect of lactate on O2 binding properties of sperm whale and horse heart myoglobins (Mb) has been investigated at moderately acid pH (i.e. pH 6.5, a condition which may be achieved in vivo under a physical effort). Addition of lactate brings about a decrease of O2 affinity (i.e. an increase of P50) in sperm whale and horse heart myoglobins. Accordingly, lactate shows a different affinity for the deoxygenated and oxygenated form, behaving as a heterotropic modulator. The lactate effect on O2 affinity appears to differ for sperm whale and horse heart Mb, δlogP50 being ≃1.0 and ≃0.4, respectively. From the kinetic viewpoint, the variation of O2 affinity for both myoglobins can be attributed mainly to a decrease of the kinetic association rate constant for ligand binding.

Original languageEnglish
Pages (from-to)16999-17001
Number of pages3
JournalJournal of Biological Chemistry
Volume271
Issue number29
DOIs
Publication statusPublished - 1996

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Myoglobin
Sperm Whale
Lactic Acid
Modulation
Horses
Physical Exertion
Kinetics
Modulators
Rate constants
Association reactions
Ligands
Acids

ASJC Scopus subject areas

  • Biochemistry

Cite this

Giardina, B., Ascenzi, P., Clementi, M. E., De Sanctis, G., Rizzi, M., & Coletta, M. (1996). Functional modulation by lactate of myoglobin. A monomeric allosteric hemoprotein. Journal of Biological Chemistry, 271(29), 16999-17001. https://doi.org/10.1074/jbc.271.29.16999

Functional modulation by lactate of myoglobin. A monomeric allosteric hemoprotein. / Giardina, Bruno; Ascenzi, Paolo; Clementi, Maria Elisabetta; De Sanctis, Giampiero; Rizzi, Menico; Coletta, Massimo.

In: Journal of Biological Chemistry, Vol. 271, No. 29, 1996, p. 16999-17001.

Research output: Contribution to journalArticle

Giardina, B, Ascenzi, P, Clementi, ME, De Sanctis, G, Rizzi, M & Coletta, M 1996, 'Functional modulation by lactate of myoglobin. A monomeric allosteric hemoprotein', Journal of Biological Chemistry, vol. 271, no. 29, pp. 16999-17001. https://doi.org/10.1074/jbc.271.29.16999
Giardina, Bruno ; Ascenzi, Paolo ; Clementi, Maria Elisabetta ; De Sanctis, Giampiero ; Rizzi, Menico ; Coletta, Massimo. / Functional modulation by lactate of myoglobin. A monomeric allosteric hemoprotein. In: Journal of Biological Chemistry. 1996 ; Vol. 271, No. 29. pp. 16999-17001.
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