Functional modulation of hepatitis B core antigen-specific T lymphocytes by an autoreactive T cell clone

C. Ferrari, F. V. Chisari, E. Ribera, A. Penna, M. U. Mondelli

Research output: Contribution to journalArticlepeer-review

Abstract

Hepatitis B core (HBc)Ag-specific T cells present in the peripheral blood of a patient with chronic active hepatitis B were expanded by co-cultivation for 7 days with rHBcAg. After cloning at 1 cell/well in the presence of PHA and IL-2, five HBcAg-specific CD4+ cloned lines were obtained. All five lines proliferated and produced IL-2, IFN-γ, and TNF in a dose-dependent fashion in response to HBcAg, but not to HBV envelope Ag. The cloned lines and derivative clones were HLA class II (DR1) restricted. All T cell clones were able to induce anti-HBc production by autologous B cells in response to HBcAg (helper effect). The proliferative response and the helper effect of the HBcAg-specific T cell lines and clones were augmented by co-cultivation with an autologous, autoreactive (HLA-DQ1 specific) T cell clone, even in the absence of HBcAg, and the autoreactive T cells directly stimulated anti-HBc secretion by autologous B cells, presumably due to the release of Ag-nonspecific factors. These findings define a model immunoregulatory circuit the physiologic significance of which remains to be determined.

Original languageEnglish
Pages (from-to)1155-1160
Number of pages6
JournalJournal of Immunology
Volume141
Issue number4
Publication statusPublished - 1988

ASJC Scopus subject areas

  • Immunology

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