Functional osteoclastogenesis: The baseline variability in blood donor precursors is not associated with age and gender

Eliana Pivetta, Bruna Wassermann, Pietro Bulian, Agostino Steffan, Alfonso Colombatti, Jerry Polesel, Paola Spessotto

Research output: Contribution to journalArticle

Abstract

Mononuclear osteoclast precursors circulate in the monocyte fraction of peripheral blood and form multinuclear cells with all osteoclastic phenotypic characteristics when cultured in the presence of macrophage colony stimulating factor (M-CSF) and receptor activator of nuclear factor kB ligand (RANKL). The method to obtain osteoclast precursors from peripheral blood is simple but the number of recovered osteoclasts is often largely insufficient for functional analyses. The original aim of this study was to develop a rapid and efficient method that could overcome the donor variability and enrich the osteoclast precursors from a small volume of peripheral blood as a basis for future clinical studies to correlate the differentiation potential of circulating osteoclast precursors with bone lesions in cancer patients. We improved the efficiency of osteoclastogenesis by reducing isolation and purification times and overcame the use of flow cytometry and immunomagnetic purification procedures. In our culture system the osteoclast number was increased severalfold and the precursors were able to reach a full differentiation within seven days of culture. Both age as well as gender differences in osteoclastogenesis efficiency were no longer evident by processing limited volume blood samples with this simple and rapid method.

Original languageEnglish
Pages (from-to)31889-31900
Number of pages12
JournalOncotarget
Volume6
Issue number31
DOIs
Publication statusPublished - 2015

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Keywords

  • Bone lesions
  • CD16
  • Isolation procedure
  • Monocytes
  • Osteoclastogenesis

ASJC Scopus subject areas

  • Oncology

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