Functional properties of neuronal nicotinic acetylcholine receptor channels expressed in transfected human cells

D. Ragozzino, S. Fucile, A. Giovannelli, F. Grassi, A. M. Mileo, M. Ballivet, S. Alemà, F. Eusebi

Research output: Contribution to journalArticlepeer-review

Abstract

To study how subunit composition affects the functional properties of neuronal nicotinic acetylcholine receptors (nAChRs), we examined the behaviour of acetylcholine (ACh)-induced single-channel currents in human BOSC 23 cells transiently transfected with various subunit cDNA combinations. For all nAChRs examined (chick and rat α 3β 4, chick α 3β 2, α 4β 2, α 4β 4, α 7 and α 8), expression levels were high enough to allow measurements of acetylcholine-evoked whole-cell currents and nicotine-elicited Ca 2+ transients as well as the functional characterization of nAChR channels. Unitary acetylcholine-evoked events of α 8 nAChR had a slope conductance of 23 pS, whereas two conductance classes (19-23 and 32-45 pS) were identified for all other nAChR channels. The mean channel open times were significantly longer for homomeric α 7 and α 8 nAChRs (6-7 ms) than for heteromeric nAChRs (1-3 ms), with the exception of α 3β 4 nAChRs (8.4 ms for rat, 7 ms for chick). At least two species of heterologously expressed nAChRs (α 3β 4 and α 3β 2) exhibited single-channel characteristics similar to those reported for native receptors. The variety of nAChR channel conductance and kinetic properties encountered in human cells transfected with nAChR subunits contributes to the functional diversity of nAChRs in nerve cells.

Original languageEnglish
Pages (from-to)480-488
Number of pages9
JournalEuropean Journal of Neuroscience
Volume9
Issue number3
DOIs
Publication statusPublished - 1997

Keywords

  • Ca transients
  • Laser confocal microscopy
  • Patch-clamp
  • Single-channel conductance
  • Transfection

ASJC Scopus subject areas

  • Neuroscience(all)

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