The potential for isolating, cultivating, manipulating and retransplanting myoblasts (satellite cells) has already been demonstrated, opening opportunities to their application, at least in the treatment of myopathies and in genic therapy. Considering previous findings obtained in vitro, which demonstrated the existence of a muscle-specific mitogen released by the macrophages, it was decided to extend the experiment to test the potential for reconstructing skeletal muscle in vivo, by means of activated macrophages and/or myoblasts. The rats used in the experiment came from the farm of the Department of Experimental Biomedical Sciences of Padua University, of Wistar stock, weighing 180-200 gms. The anesthetized animals were admitted to surgical removal of the tibialis anterior muscle and then muscle cells were inoculated into the resulting cavity, according to the following procedure: a) removal of tibialis anterior muscle (-TA) b) removal of tibialis anterior muscle and local inoculation of myoblasts (-TA, +myoblasts) c) removal of tibialis anterior muscle and local inoculation of myoblasts and macrophages (-TA, +myoblasts and +macrophages). d) removal of tibialis anterior muscle and local inoculation of myoblasts in a bed composed of slices of liver. Seven days later the animals were sacrificed. The tissue present in the cavity was removed and analysed using embryonal antimyosin monoclonal antibodies to determine the degree of muscle regeneration and/or integration of the transplanted myoblasts. Findings show that seven days after the muscle cavity is filled with liver tissue and then inoculated with myoblasts, the resulting tissue mass completely fills the preformed cavity and, on microscopic examination, presents numerous fibres positive to embryonal antimyosin monoclonal antibody, i.e. is muscular tissue.
|Translated title of the contribution||Functional reconstruction of skeletal muscle in animals by transplanting myoblasts activated in vitro|
|Number of pages||7|
|Journal||Rivista Italiana di Chirurgia Plastica|
|Publication status||Published - 1999|
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