Functional role of transient conformations: Rediscovering "chronosteric effects" thirty years later

Paolo Ascenzi, Stefano Gianni

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Proteins are dynamic entities that exert, in some cases, their functions via complex pathways, involving active transient species. This phenomenon was highlighted for the first time in 1983 by Antonini et al. (J. Biol. Chem. 258, 4676-4678), who demonstrated that at least one intermediate occurring in the formation of the bovine β-trypsin-Kunitz inhibitor complex displayed catalytic properties different from those of the active enzyme and of the inactive enzyme-inhibitor adduct. Since it was impossible to explain this phenomenon in terms of static three-dimensional structures, the term "chronosteric effects" was coined to capture the observation that transient species are relevant to protein function(s). Here, some recent results on the folding and function of proteins are reported on the light of chronosteric effects.

Original languageEnglish
Pages (from-to)836-844
Number of pages9
JournalIUBMB Life
Volume65
Issue number10
DOIs
Publication statusPublished - Oct 2013

Fingerprint

Conformations
Trypsin Inhibitors
Protein Folding
Enzyme Inhibitors
Proteins
Light
Trypsin
Enzymes

Keywords

  • chronosteric effects
  • folding intermediates
  • human estrogen receptors
  • human serum heme-albumin
  • kinetics
  • protein folding
  • rapid signals and transcription activity
  • serine proteinase inhibition
  • transient catalytic properties
  • transient heme-based reactivity

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Clinical Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Functional role of transient conformations : Rediscovering "chronosteric effects" thirty years later. / Ascenzi, Paolo; Gianni, Stefano.

In: IUBMB Life, Vol. 65, No. 10, 10.2013, p. 836-844.

Research output: Contribution to journalArticle

Ascenzi, Paolo ; Gianni, Stefano. / Functional role of transient conformations : Rediscovering "chronosteric effects" thirty years later. In: IUBMB Life. 2013 ; Vol. 65, No. 10. pp. 836-844.
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