Functional study of lipids of PNH red cell membranes: Susceptibility of liposomes to reactive lysis

F. Tedesco, I. Kahane, A. Zanella, A. M. Giovanetti, G. Sirchia

Research output: Contribution to journalArticlepeer-review

Abstract

Lipids extracted with chloroform-methanol from red blood cell membranes of 7 PNH and 13 control subjects were used for the preparation of liposomes, which were then examined with the reactive lysis test. PNH liposomes lysed to a higher extent than control liposomes as indicated by the higher dilution of the limiting complement reagent that was necessary to lyse 50% of the PNH liposomes. A similar finding was also observed with liposomes made of lipids from AET-treated red cells. The enhanced reactive lysis can be attributed to the polar lipid fraction, as indicated by the increased lysis of hybrid liposomes prepared from the polar lipids extracted from PNH erythrocyte membrane and lipids extracted from normal erythrocyte membrane. The increased susceptibility to reactive lysis does not seem to be specific of PNH liposomes, since it was also observed with liposomes prepared from lipids of red cells from β-thalassemia major and autoimmune hemolytic disease.

Original languageEnglish
Pages (from-to)900-905
Number of pages6
JournalBlood
Volume57
Issue number5
Publication statusPublished - 1981

ASJC Scopus subject areas

  • Hematology

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