Further studies on the gangliosidic nature of the cholinergic-specific antigen, Chol-1

Attilia Giuliani, Emanuela Calappi, Edilio Borroni, Victor P. Whittaker, Sandro Sonnino, Guido Tettamanti

Research output: Contribution to journalArticle

Abstract

The antigen designated as Chol-1β, detected by an antiserum specific for cholinergic neurons, has been purified to homogeneity from ganglioside mixtures extracted from Torpedo electric organ and pig brain. The final products from the two sources behaved identically in a wide range of tests and gave coincident immunopositive and Ehrlich-positive spots after thin layer chromatography in seven different solvent systems; they were thus considered to be identical and to constitute a single, pure chemical species. Gas-chromatographic analysis revealed the presence of long-chain bases, glucose, galactose, N-acetylgalactosamine, and sialic acid in integral molar ratios of 1:1:2:1:3; the compound's reactivity to cholera toxin after Vibrio cholerae sialidase treatment on thin layer chromatography and the recovery of GM1 as sole product of exhaustive sialidase treatment identified it as a member of the gangliotetrahexosyl series. From the products of partial enzymatic desialylation and treatment with β-galactosidase and a comparison of the compound's immunoreactivity to anti-Chol-1 antisera with that of other trisialogangliosides of defined molecular structure, we were able to assign a disialosyl residue α-Neu5Ac-(2 → 8)-α-Neu5Ac-(2 → 3)- to the inner galactose, and we suggest Ga1NAc as a possible site of linkage of the third sialic acid.

Original languageEnglish
Pages (from-to)211-216
Number of pages6
JournalArchives of Biochemistry and Biophysics
Volume280
Issue number1
DOIs
Publication statusPublished - 1990

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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  • Cite this

    Giuliani, A., Calappi, E., Borroni, E., Whittaker, V. P., Sonnino, S., & Tettamanti, G. (1990). Further studies on the gangliosidic nature of the cholinergic-specific antigen, Chol-1. Archives of Biochemistry and Biophysics, 280(1), 211-216. https://doi.org/10.1016/0003-9861(90)90538-A