G protein-coupled receptor kinase 2 activity impairs cardiac glucose uptake and promotes insulin resistance after myocardial ischemia

Michele Ciccarelli, J. Kurt Chuprun, Giuseppe Rengo, Erhe Gao, Zhengyu Wei, Raymond J. Peroutka, Jessica I. Gold, Anna Gumpert, Mai Chen, Nicholas J. Otis, Gerald W. Dorn, Bruno Trimarco, Guido Iaccarino, Walter J. Koch

Research output: Contribution to journalArticle

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Abstract

Background-: Alterations in cardiac energy metabolism downstream of neurohormonal stimulation play a crucial role in the pathogenesis of heart failure. The chronic adrenergic stimulation that accompanies heart failure is a signaling abnormality that leads to the upregulation of G protein-coupled receptor kinase 2 (GRK2), which is pathological in the myocyte during disease progression in part owing to uncoupling of the β-adrenergic receptor system. In this study, we explored the possibility that enhanced GRK2 expression and activity, as seen during heart failure, can negatively affect cardiac metabolism as part of its pathogenic profile. Methods and Results-: Positron emission tomography studies revealed in transgenic mice that cardiac-specific overexpression of GRK2 negatively affected cardiac metabolism by inhibiting glucose uptake and desensitization of insulin signaling, which increases after ischemic injury and precedes heart failure development. Mechanistically, GRK2 interacts with and directly phosphorylates insulin receptor substrate-1 in cardiomyocytes, causing insulin-dependent negative signaling feedback, including inhibition of membrane translocation of the glucose transporter GLUT4. This identifies insulin receptor substrate-1 as a novel nonreceptor target for GRK2 and represents a new pathological mechanism for this kinase in the failing heart. Importantly, inhibition of GRK2 activity prevents postischemic defects in myocardial insulin signaling and improves cardiac metabolism via normalized glucose uptake, which appears to participate in GRK2-targeted prevention of heart failure. Conclusions-: Our data provide novel insights into how GRK2 is pathological in the injured heart. Moreover, it appears to be a critical mechanistic link within neurohormonal crosstalk governing cardiac contractile signaling/function through β-adrenergic receptors and metabolism through the insulin receptor.

Original languageEnglish
Pages (from-to)1953-1962
Number of pages10
JournalCirculation
Volume123
Issue number18
DOIs
Publication statusPublished - May 10 2011

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G-Protein-Coupled Receptor Kinase 2
Myocardial Ischemia
Insulin Resistance
Glucose
Heart Failure
Insulin Receptor Substrate Proteins
Insulin
Adrenergic Receptors
Facilitative Glucose Transport Proteins
Insulin Receptor
Cardiac Myocytes
Positron-Emission Tomography
Adrenergic Agents
Muscle Cells
Energy Metabolism
Transgenic Mice
Disease Progression
Phosphotransferases
Up-Regulation

Keywords

  • glucose
  • heart failure
  • myocardial ischemia
  • positron-emission tomography

ASJC Scopus subject areas

  • Physiology (medical)
  • Cardiology and Cardiovascular Medicine

Cite this

G protein-coupled receptor kinase 2 activity impairs cardiac glucose uptake and promotes insulin resistance after myocardial ischemia. / Ciccarelli, Michele; Chuprun, J. Kurt; Rengo, Giuseppe; Gao, Erhe; Wei, Zhengyu; Peroutka, Raymond J.; Gold, Jessica I.; Gumpert, Anna; Chen, Mai; Otis, Nicholas J.; Dorn, Gerald W.; Trimarco, Bruno; Iaccarino, Guido; Koch, Walter J.

In: Circulation, Vol. 123, No. 18, 10.05.2011, p. 1953-1962.

Research output: Contribution to journalArticle

Ciccarelli, M, Chuprun, JK, Rengo, G, Gao, E, Wei, Z, Peroutka, RJ, Gold, JI, Gumpert, A, Chen, M, Otis, NJ, Dorn, GW, Trimarco, B, Iaccarino, G & Koch, WJ 2011, 'G protein-coupled receptor kinase 2 activity impairs cardiac glucose uptake and promotes insulin resistance after myocardial ischemia', Circulation, vol. 123, no. 18, pp. 1953-1962. https://doi.org/10.1161/CIRCULATIONAHA.110.988642
Ciccarelli, Michele ; Chuprun, J. Kurt ; Rengo, Giuseppe ; Gao, Erhe ; Wei, Zhengyu ; Peroutka, Raymond J. ; Gold, Jessica I. ; Gumpert, Anna ; Chen, Mai ; Otis, Nicholas J. ; Dorn, Gerald W. ; Trimarco, Bruno ; Iaccarino, Guido ; Koch, Walter J. / G protein-coupled receptor kinase 2 activity impairs cardiac glucose uptake and promotes insulin resistance after myocardial ischemia. In: Circulation. 2011 ; Vol. 123, No. 18. pp. 1953-1962.
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AU - Chuprun, J. Kurt

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AU - Wei, Zhengyu

AU - Peroutka, Raymond J.

AU - Gold, Jessica I.

AU - Gumpert, Anna

AU - Chen, Mai

AU - Otis, Nicholas J.

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AU - Trimarco, Bruno

AU - Iaccarino, Guido

AU - Koch, Walter J.

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N2 - Background-: Alterations in cardiac energy metabolism downstream of neurohormonal stimulation play a crucial role in the pathogenesis of heart failure. The chronic adrenergic stimulation that accompanies heart failure is a signaling abnormality that leads to the upregulation of G protein-coupled receptor kinase 2 (GRK2), which is pathological in the myocyte during disease progression in part owing to uncoupling of the β-adrenergic receptor system. In this study, we explored the possibility that enhanced GRK2 expression and activity, as seen during heart failure, can negatively affect cardiac metabolism as part of its pathogenic profile. Methods and Results-: Positron emission tomography studies revealed in transgenic mice that cardiac-specific overexpression of GRK2 negatively affected cardiac metabolism by inhibiting glucose uptake and desensitization of insulin signaling, which increases after ischemic injury and precedes heart failure development. Mechanistically, GRK2 interacts with and directly phosphorylates insulin receptor substrate-1 in cardiomyocytes, causing insulin-dependent negative signaling feedback, including inhibition of membrane translocation of the glucose transporter GLUT4. This identifies insulin receptor substrate-1 as a novel nonreceptor target for GRK2 and represents a new pathological mechanism for this kinase in the failing heart. Importantly, inhibition of GRK2 activity prevents postischemic defects in myocardial insulin signaling and improves cardiac metabolism via normalized glucose uptake, which appears to participate in GRK2-targeted prevention of heart failure. Conclusions-: Our data provide novel insights into how GRK2 is pathological in the injured heart. Moreover, it appears to be a critical mechanistic link within neurohormonal crosstalk governing cardiac contractile signaling/function through β-adrenergic receptors and metabolism through the insulin receptor.

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