G6PD is indispensable for erythropoiesis after the embryonic-adult hemoglobin switch

Francesca Paglialunga, Annalisa Fico, Ingram Iaccarino, Rosario Notaro, Lucio Luzzatto, Giuseppe Martini, Stefania Filosa

Research output: Contribution to journalArticlepeer-review

Abstract

Glucose 6-phosphate dehydrogenase (G6PD) (EC 1.1.1.42) is an essential enzyme for the rapid production of NADPH, as required on exposure to oxidative stress. Mouse embryonic stem (ES) cells can produce all embryonic and fetal/adult cell types. By studying the in vitro differentiation of embryoid bodies produced from G6pdΔ ES cells that are totally unable to produce G6PD protein, we found that these cells are able to differentiate into mesodermal cells, cardiomyocytes, hepatocytes, and primitive erythroid cells. However, we show here that, after the hemoglobin switch has taken place, definitive erythrocytes die by apoptosis. This apoptotic death is delayed by reducing agents and by a caspase inhibitor, but it is prevented only by the restoration of G6PD activity. Thus, G6PD proves indispensable for definitive erythropoiesis.

Original languageEnglish
Pages (from-to)3148-3152
Number of pages5
JournalBlood
Volume104
Issue number10
DOIs
Publication statusPublished - Nov 15 2004

ASJC Scopus subject areas

  • Hematology

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