GABAergic interneurons and neuropil of the intralaminar thalamus: an immunohistochemical study in the rat and the cat, with notes in the monkey

M. Bentivoglio, R. Spreafico, D. Minciacchi, G. Macchi

Research output: Contribution to journalArticle

Abstract

Immunohistochemistry using antibodies to glutamic acid decarboxylase (GAD) was used to investigate the intralaminar nuclei of the thalamus in rat, cat and monkey. Antibodies to gamma aminobutyric acid (GABA) were also used in the cat. Intralaminar immunoreactive cell bodies were not detected in the rat, but were clearly present in cat and monkey. In the latter species, GABA- or GAD-immunopositive perikarya were distributed throughout the anterior intralaminar nuclei, whereas in the posterior intralaminar complex they prevailed in the lateral part of the centre median nucleus and around the fasciculus retrofiexus. Measurements of the area of immunostained intralaminar cell bodies in cat and monkey indicated that they are represented by small neurons. Experiments in the cat, based on retrograde tracers injections involving large sectors of the frontal and parietal cortices and the head of the caudate nucleus, revealed that the GABA- or GAD-immunoreactive cells and the retrogradely labeled projection neurons represented two separate intralaminar cell populations, although the latter also included small cells. Considerable differences were observed in the immunoreactive GABAergic neuropil of the anterior and posterior intralaminar nuclei. Clusters of densely packed bouton-like immunoreactive elements were detected in the former structures in the rat, cat and monkey, and were especially evident in the central lateral nucleus; immunopositive varicose fibers and puncta were diffusely distributed in the posterior intralaminar structures. Taken together with data from the literature, the present findings indicate that in cat and monkey local circuit inhibitory cells regulate not only the activity of principal thalamic nuclei which project densely upon restricted cortical fields, but also of the intralaminar structures which are widely connected with the cerebral cortex and the striatum. Regional variations in the distribution of GABAergic fibers and terminals suggest major differences in the organization of inhibitory circuits and synaptic arrangements of the anterior and posterior intralaminar thalamus.

Original languageEnglish
Pages (from-to)85-95
Number of pages11
JournalExperimental Brain Research
Volume87
Issue number1
DOIs
Publication statusPublished - Oct 1991

Fingerprint

Neuropil
Interneurons
Thalamus
Haplorhini
Cats
Glutamate Decarboxylase
gamma-Aminobutyric Acid
Intralaminar Thalamic Nuclei
Neurons
Thalamic Nuclei
Parietal Lobe
Antibodies
Caudate Nucleus
Frontal Lobe
Cerebral Cortex
Immunohistochemistry
Injections
Population

Keywords

  • GABA
  • Immunohistochemistry
  • Inhibition
  • Intralaminar nuclei
  • Thalamus

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

GABAergic interneurons and neuropil of the intralaminar thalamus : an immunohistochemical study in the rat and the cat, with notes in the monkey. / Bentivoglio, M.; Spreafico, R.; Minciacchi, D.; Macchi, G.

In: Experimental Brain Research, Vol. 87, No. 1, 10.1991, p. 85-95.

Research output: Contribution to journalArticle

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