GABAA receptor cell surface number and subunit stability are regulated by the ubiquitin-like protein Plic-1

Fiona K. Bedford, Josef T. Kittler, Emilie Muller, Philip Thomas, Julia M. Uren, Daniela Merlo, William Wisden, Antoine Triller, Trevor G. Smart, Stephen J. Moss

Research output: Contribution to journalArticle

187 Citations (Scopus)

Abstract

Controlling the number of functional γ-aminobutyric acid A (GABAA) receptors in neuronal membranes is a crucial factor for the efficacy of inhibitory neurotransmission. Here we describe the direct interaction of GABAA receptors with the ubiquitin-like protein Plic-1. Furthermore, Plic-1 is enriched at inhibitory synapses and is associated with subsynaptic membranes. Functionally, Plic-1 facilitates GABAA receptor cell surface expression without affecting the rate of receptor internalization. Plic-1 also enhances the stability of intracellular GABAA receptor subunits, increasing the number of receptors available for insertion into the plasma membrane. Our study identifies a previously unknown role for Plic-1, a modulation of GABAA receptor cell surface number, which suggests that Plic-1 facilitates accumulation of these receptors in dendritic membranes.

Original languageEnglish
Pages (from-to)908-916
Number of pages9
JournalNature Neuroscience
Volume4
Issue number9
DOIs
Publication statusPublished - 2001

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Ubiquitins
GABA-A Receptors
Cell Count
Membranes
Aminobutyrates
Synaptic Transmission
Synapses
Cell Membrane

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

GABAA receptor cell surface number and subunit stability are regulated by the ubiquitin-like protein Plic-1. / Bedford, Fiona K.; Kittler, Josef T.; Muller, Emilie; Thomas, Philip; Uren, Julia M.; Merlo, Daniela; Wisden, William; Triller, Antoine; Smart, Trevor G.; Moss, Stephen J.

In: Nature Neuroscience, Vol. 4, No. 9, 2001, p. 908-916.

Research output: Contribution to journalArticle

Bedford, FK, Kittler, JT, Muller, E, Thomas, P, Uren, JM, Merlo, D, Wisden, W, Triller, A, Smart, TG & Moss, SJ 2001, 'GABAA receptor cell surface number and subunit stability are regulated by the ubiquitin-like protein Plic-1', Nature Neuroscience, vol. 4, no. 9, pp. 908-916. https://doi.org/10.1038/nn0901-908
Bedford, Fiona K. ; Kittler, Josef T. ; Muller, Emilie ; Thomas, Philip ; Uren, Julia M. ; Merlo, Daniela ; Wisden, William ; Triller, Antoine ; Smart, Trevor G. ; Moss, Stephen J. / GABAA receptor cell surface number and subunit stability are regulated by the ubiquitin-like protein Plic-1. In: Nature Neuroscience. 2001 ; Vol. 4, No. 9. pp. 908-916.
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