GABAA receptor cell surface number and subunit stability are regulated by the ubiquitin-like protein Plic-1

Fiona K. Bedford, Josef T. Kittler, Emilie Muller, Philip Thomas, Julia M. Uren, Daniela Merlo, William Wisden, Antoine Triller, Trevor G. Smart, Stephen J. Moss

Research output: Contribution to journalArticle

Abstract

Controlling the number of functional γ-aminobutyric acid A (GABAA) receptors in neuronal membranes is a crucial factor for the efficacy of inhibitory neurotransmission. Here we describe the direct interaction of GABAA receptors with the ubiquitin-like protein Plic-1. Furthermore, Plic-1 is enriched at inhibitory synapses and is associated with subsynaptic membranes. Functionally, Plic-1 facilitates GABAA receptor cell surface expression without affecting the rate of receptor internalization. Plic-1 also enhances the stability of intracellular GABAA receptor subunits, increasing the number of receptors available for insertion into the plasma membrane. Our study identifies a previously unknown role for Plic-1, a modulation of GABAA receptor cell surface number, which suggests that Plic-1 facilitates accumulation of these receptors in dendritic membranes.

Original languageEnglish
Pages (from-to)908-916
Number of pages9
JournalNature Neuroscience
Volume4
Issue number9
DOIs
Publication statusPublished - 2001

ASJC Scopus subject areas

  • Neuroscience(all)

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    Bedford, F. K., Kittler, J. T., Muller, E., Thomas, P., Uren, J. M., Merlo, D., Wisden, W., Triller, A., Smart, T. G., & Moss, S. J. (2001). GABAA receptor cell surface number and subunit stability are regulated by the ubiquitin-like protein Plic-1. Nature Neuroscience, 4(9), 908-916. https://doi.org/10.1038/nn0901-908