GABP complex regulates transcription of eIF6 (p27BBP), an essential trans-acting factor in ribosome biogenesis

Alessandra Donadini, Francesca Giacopelli, Roberto Ravazzolo, Valentina Gandin, Pier Carlo Marchisio, Stefano Biffo

Research output: Contribution to journalArticlepeer-review

Abstract

Eukaryotic initiation factor 6 (eIF6, alias p27BBP) is required for the biogenesis of 60S ribosomal subunits. eIF6 expression levels are tightly regulated in vivo, where they correlate with cellular growth. We analyzed how transcriptional regulation of eIF6 is achieved. We show that the human eIF6 promoter contains consensus sites for the GABP (GA-binding protein) transcription factor complex. Functional analysis of GABP consensus sequences by point mutations, EMSA (electrophoretic mobility shift assay) and a dominant negative mutant indicates that GABP is essential for eIF6 promoter activity. These data strengthen the hypothesis that GABP is a global regulator of ribosome synthesis.

Original languageEnglish
Pages (from-to)1983-1987
Number of pages5
JournalFEBS Letters
Volume580
Issue number8
DOIs
Publication statusPublished - Apr 3 2006

Keywords

  • Beta binding protein (p27BBP)
  • ITGB4PB gene
  • Nucleolus
  • Translation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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