GAD and IA-2 autoantibody detection in type 1 diabetic patient saliva

Claudio Tiberti, Blegina Shashaj, Antonella Verrienti, Elio Giancarlo Vecci, Federica Lucantoni, Donata Masotti, Susanna Morano, Nicoletta Sulli, Francesco Dotta

Research output: Contribution to journalArticle

Abstract

Some attempts have been made in assaying glutamic-acid decarboxylase autoantibodies (GADA) in type 1 diabetic patient (T1DM) saliva. However, these salivary assays did not show sufficient sensitivity and specificity in comparison to serum assays. In this study we evaluated the ability of a fluid-phase 35S-radioimmunoassay to detect GADA and tyrosine phosphatase 2 autoantibodies (IA-2A) in 70 T1DM, 24 T1DM first degree relatives (FDR) and 76 healthy subject saliva. Paired saliva and serum samples were collected from each subject and analyzed. GADA were detected in 45/70 (64.3%) sera and 43/70 (61.4%) T1DM saliva, respectively. IA-2A were detected in 33/70 (47.1%) sera and 30/70 (42.9%) T1DM saliva, respectively. All FDR serum/saliva samples were autoantibody negative. In conclusion, we here report that GADA and IA-2A are detectable with high sensitivity and specificity in human saliva, a specimen which can be easily collected by non-invasive procedures and may represent a reliable tool for the study of T1DM autoimmunity.

Original languageEnglish
Pages (from-to)271-276
Number of pages6
JournalClinical Immunology
Volume131
Issue number2
DOIs
Publication statusPublished - May 2009

Fingerprint

Saliva
Glutamate Decarboxylase
Autoantibodies
Serum
Sensitivity and Specificity
Aptitude
ICA512 autoantibody
Autoimmunity
Phosphoric Monoester Hydrolases
Radioimmunoassay
Tyrosine
Healthy Volunteers

Keywords

  • GAD autoantibodies
  • Human saliva
  • IA-2 autoantibodies
  • Type 1 diabetes

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Tiberti, C., Shashaj, B., Verrienti, A., Vecci, E. G., Lucantoni, F., Masotti, D., ... Dotta, F. (2009). GAD and IA-2 autoantibody detection in type 1 diabetic patient saliva. Clinical Immunology, 131(2), 271-276. https://doi.org/10.1016/j.clim.2008.12.002

GAD and IA-2 autoantibody detection in type 1 diabetic patient saliva. / Tiberti, Claudio; Shashaj, Blegina; Verrienti, Antonella; Vecci, Elio Giancarlo; Lucantoni, Federica; Masotti, Donata; Morano, Susanna; Sulli, Nicoletta; Dotta, Francesco.

In: Clinical Immunology, Vol. 131, No. 2, 05.2009, p. 271-276.

Research output: Contribution to journalArticle

Tiberti, C, Shashaj, B, Verrienti, A, Vecci, EG, Lucantoni, F, Masotti, D, Morano, S, Sulli, N & Dotta, F 2009, 'GAD and IA-2 autoantibody detection in type 1 diabetic patient saliva', Clinical Immunology, vol. 131, no. 2, pp. 271-276. https://doi.org/10.1016/j.clim.2008.12.002
Tiberti, Claudio ; Shashaj, Blegina ; Verrienti, Antonella ; Vecci, Elio Giancarlo ; Lucantoni, Federica ; Masotti, Donata ; Morano, Susanna ; Sulli, Nicoletta ; Dotta, Francesco. / GAD and IA-2 autoantibody detection in type 1 diabetic patient saliva. In: Clinical Immunology. 2009 ; Vol. 131, No. 2. pp. 271-276.
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