Abstract
Gadolinium deposition in tissue is linked to nephrogenic systemic fibrosis (NSF): a rare disorder occurring in patients with severe chronic kidney disease and associated with administration of Gd-based contrast agents (GBCAs) for Magnetic Resonance Imaging (MRI). It is suggested that the GBCAs prolonged permanence in blood in these patients may result in a Gd precipitation in peripheral or central organs, where it initiates a fibrotic process. In this study we investigated new sites of retention/precipitation of Gd in a mouse model of renal disease (5/6 nephrectomy) receiving two doses (closely after each other) of a linear GBCA. Two commercial GBCAs (Omniscan® and Magnevist®) were administered at doses slightly higher than those used in clinical practice (0.7 mmol/kg body weight, each). The animals were sacrificed one month after the last administration and the explanted organs (kidney, liver, femur, dorsal skin, teeth) were analysed by X-ray fluorescence (XRF) at two synchrotron facilities. The XRF analysis with a millimetre-sized beam at the SYRMEP beamline (Elettra, Italy) produced no detectable levels of Gd in the examined tissues, with the notable exception of the incisors of the nephrectomised mice. The XRF analyses at sub-micron resolution performed at ID21 (ESRF, France) allowed to clearly localize Gd in the periodontal ligaments of teeth both from Omniscan® and Magnevist® treated nephrectomised mice. The latter results were further confirmed by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). The study prompts that prolonged permanence of GBCAs in blood may result in Gd retention in this particular muscular tissue, opening possibilities for diagnostic applications at this level when investigating Gd-related toxicities.
| Original language | English |
|---|---|
| Pages (from-to) | 157-167 |
| Number of pages | 11 |
| Journal | Toxicology Letters |
| Volume | 301 |
| DOIs | |
| Publication status | Published - Feb 1 2019 |
Fingerprint
Keywords
- 5/6 nephrectomy
- Gadolinium
- GBCAs
- MRI contrast agent
- Periodontal ligament
- XRF
ASJC Scopus subject areas
- Toxicology
Cite this
Gadolinium tissue deposition in the periodontal ligament of mice with reduced renal function exposed to Gd-based contrast agents. / Delfino, Riccarda; Biasotto, Matteo; Candido, Riccardo; Altissimo, Matteo; Stebel, Marco; Salomè, Murielle; van Elteren, Johannes T.; Vogel Mikuš, Katarina; Zennaro, Cristina; Šala, Martin; Addobbati, Riccardo; Tromba, Giuliana; Pascolo, Lorella.
In: Toxicology Letters, Vol. 301, 01.02.2019, p. 157-167.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Gadolinium tissue deposition in the periodontal ligament of mice with reduced renal function exposed to Gd-based contrast agents
AU - Delfino, Riccarda
AU - Biasotto, Matteo
AU - Candido, Riccardo
AU - Altissimo, Matteo
AU - Stebel, Marco
AU - Salomè, Murielle
AU - van Elteren, Johannes T.
AU - Vogel Mikuš, Katarina
AU - Zennaro, Cristina
AU - Šala, Martin
AU - Addobbati, Riccardo
AU - Tromba, Giuliana
AU - Pascolo, Lorella
PY - 2019/2/1
Y1 - 2019/2/1
N2 - Gadolinium deposition in tissue is linked to nephrogenic systemic fibrosis (NSF): a rare disorder occurring in patients with severe chronic kidney disease and associated with administration of Gd-based contrast agents (GBCAs) for Magnetic Resonance Imaging (MRI). It is suggested that the GBCAs prolonged permanence in blood in these patients may result in a Gd precipitation in peripheral or central organs, where it initiates a fibrotic process. In this study we investigated new sites of retention/precipitation of Gd in a mouse model of renal disease (5/6 nephrectomy) receiving two doses (closely after each other) of a linear GBCA. Two commercial GBCAs (Omniscan® and Magnevist®) were administered at doses slightly higher than those used in clinical practice (0.7 mmol/kg body weight, each). The animals were sacrificed one month after the last administration and the explanted organs (kidney, liver, femur, dorsal skin, teeth) were analysed by X-ray fluorescence (XRF) at two synchrotron facilities. The XRF analysis with a millimetre-sized beam at the SYRMEP beamline (Elettra, Italy) produced no detectable levels of Gd in the examined tissues, with the notable exception of the incisors of the nephrectomised mice. The XRF analyses at sub-micron resolution performed at ID21 (ESRF, France) allowed to clearly localize Gd in the periodontal ligaments of teeth both from Omniscan® and Magnevist® treated nephrectomised mice. The latter results were further confirmed by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). The study prompts that prolonged permanence of GBCAs in blood may result in Gd retention in this particular muscular tissue, opening possibilities for diagnostic applications at this level when investigating Gd-related toxicities.
AB - Gadolinium deposition in tissue is linked to nephrogenic systemic fibrosis (NSF): a rare disorder occurring in patients with severe chronic kidney disease and associated with administration of Gd-based contrast agents (GBCAs) for Magnetic Resonance Imaging (MRI). It is suggested that the GBCAs prolonged permanence in blood in these patients may result in a Gd precipitation in peripheral or central organs, where it initiates a fibrotic process. In this study we investigated new sites of retention/precipitation of Gd in a mouse model of renal disease (5/6 nephrectomy) receiving two doses (closely after each other) of a linear GBCA. Two commercial GBCAs (Omniscan® and Magnevist®) were administered at doses slightly higher than those used in clinical practice (0.7 mmol/kg body weight, each). The animals were sacrificed one month after the last administration and the explanted organs (kidney, liver, femur, dorsal skin, teeth) were analysed by X-ray fluorescence (XRF) at two synchrotron facilities. The XRF analysis with a millimetre-sized beam at the SYRMEP beamline (Elettra, Italy) produced no detectable levels of Gd in the examined tissues, with the notable exception of the incisors of the nephrectomised mice. The XRF analyses at sub-micron resolution performed at ID21 (ESRF, France) allowed to clearly localize Gd in the periodontal ligaments of teeth both from Omniscan® and Magnevist® treated nephrectomised mice. The latter results were further confirmed by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). The study prompts that prolonged permanence of GBCAs in blood may result in Gd retention in this particular muscular tissue, opening possibilities for diagnostic applications at this level when investigating Gd-related toxicities.
KW - 5/6 nephrectomy
KW - Gadolinium
KW - GBCAs
KW - MRI contrast agent
KW - Periodontal ligament
KW - XRF
UR - http://www.scopus.com/inward/record.url?scp=85057478686&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85057478686&partnerID=8YFLogxK
U2 - 10.1016/j.toxlet.2018.11.014
DO - 10.1016/j.toxlet.2018.11.014
M3 - Article
C2 - 30476537
AN - SCOPUS:85057478686
VL - 301
SP - 157
EP - 167
JO - Toxicology Letters
JF - Toxicology Letters
SN - 0378-4274
ER -