Gain of FGF4 is a frequent event in KIT/PDGFRA/SDH/RAS-P WT GIST

Milena Urbini, Valentina Indio, Giuseppe Tarantino, Gloria Ravegnini, Sabrina Angelini, Margherita Nannini, Maristella Saponara, Donatella Santini, Claudio Ceccarelli, Michelangelo Fiorentino, Bruno Vincenzi, Elena Fumagalli, Paolo Giovanni Casali, Giovanni Grignani, Andrea Pession, Andrea Ardizzoni, Annalisa Astolfi, Maria Abbondanza Pantaleo

Research output: Contribution to journalArticle

Abstract

Gastrointestinal stromal tumors (GIST) lacking mutations in KIT/PDGFRA or RAS pathways and retaining an intact SDH complex are usually referred to as KIT/PDGFRA/SDH/RAS-P WT GIST or more simply quadruple WT GIST (~5% of all GIST). Despite efforts made, no recurrent genetic event in quadruple WT GIST has been identified so far. To further investigate this disease, we performed high throughput copy number analysis on quadruple WT GIST specimens identifying a recurrent focal gain in band 11q13.3 (involving FGF3/FGF4) in 6/8 cases. This event was not found in the other molecular GIST subgroups. FGF3/FGF4 duplication was associated with high expression of FGF4, both at mRNA and protein level, a growth factor normally not expressed in adult tissues or in KIT/PDGFRA-mutated GIST. FGFR1 was found to be the predominant FGF receptor expressed and phosphorylation of AKT was detected, suggesting that a FGF4-FGFR1 autocrine loop could stimulate downstream signaling in quadruple WT GIST. Together with the recent reports of quadruple WT cases carrying FGFR1 activating alterations, these findings strengthen the hypothesis of a potential involvement of FGFR pathway deregulation in quadruple WT GIST, which may represent a rationale for novel therapeutic approaches.

Original languageEnglish
Pages (from-to)636-642
Number of pages7
JournalGenes Chromosomes and Cancer
Volume58
Issue number9
DOIs
Publication statusPublished - Sep 2019

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Keywords

  • FGF3/FGF4
  • FGFR inhibitors
  • FGFR1
  • gastrointestinal stromal tumours
  • KIT/PDGFRA/SDH/RAS-P WT
  • quadruple WT

ASJC Scopus subject areas

  • Genetics
  • Cancer Research

Cite this

Urbini, M., Indio, V., Tarantino, G., Ravegnini, G., Angelini, S., Nannini, M., Saponara, M., Santini, D., Ceccarelli, C., Fiorentino, M., Vincenzi, B., Fumagalli, E., Casali, P. G., Grignani, G., Pession, A., Ardizzoni, A., Astolfi, A., & Pantaleo, M. A. (2019). Gain of FGF4 is a frequent event in KIT/PDGFRA/SDH/RAS-P WT GIST. Genes Chromosomes and Cancer, 58(9), 636-642. https://doi.org/10.1002/gcc.22753