Gain-of-function mutations in sodium channel NaV1.9 in painful neuropathy

Jianying Huang, Chongyang Han, Mark Estacion, Dymtro Vasylyev, Janneke G J Hoeijmakers, Monique M. Gerrits, Lynda Tyrrell, Giuseppe Lauria, Catharina G. Faber, Sulayman D. Dib-Hajj, Ingemar S J Merkies, Stephen G. Waxman

Research output: Contribution to journalArticle

131 Citations (Scopus)

Abstract

Sodium channel Nav1.9 is expressed in peripheral nociceptive neurons, as well as visceral afferents, and has been shown to act as a threshold channel. Painful peripheral neuropathy represents a significant public health challenge and may involve gain-of-function variants in sodium channels that are preferentially expressed in peripheral sensory neurons. Although gain-of-function variants of peripheral sodium channels Nav1.7 and Nav1.8 have recently been found in painful small fibre neuropathy, the aetiology of peripheral neuropathy in many cases remains unknown. We evaluated 459 patients who were referred for possible painful peripheral neuropathy, and confirmed the diagnosis of small fibre neuropathy in a cohort of 393 patients (369 patients with pure small fibre neuropathy, and small fibre neuropathy together with large fibre involvement in an additional 24 patients). From this cohort of 393 patients with peripheral neuropathy, we sequenced SCN11A in 345 patients without mutations in SCN9A and SCN10A, and found eight variants in 12 patients. Functional profiling by electrophysiological recordings showed that these Nav1.9 mutations confer gain-of-function attributes to the channel, depolarize resting membrane potential of dorsal root ganglion neurons, enhance spontaneous firing, and increase evoked firing of these neurons. Our data show, for the first time, missense mutations of Nav1.9 in individuals with painful peripheral neuropathy. These genetic and functional observations identify missense mutations of Nav1.9 as a cause of painful peripheral neuropathy.

Original languageEnglish
Pages (from-to)1627-1642
Number of pages16
JournalBrain
Volume137
Issue number6
DOIs
Publication statusPublished - 2014

Fingerprint

Sodium Channels
Peripheral Nervous System Diseases
Mutation
Missense Mutation
Visceral Afferents
Neurons
Nociceptors
Spinal Ganglia
Sensory Receptor Cells
Painful Neuropathy
Membrane Potentials
Public Health
Fiber
Small Fiber Neuropathy
Neuron

Keywords

  • channelopathy
  • current-clamp
  • DRG
  • sensory neurons
  • voltage-clamp

ASJC Scopus subject areas

  • Clinical Neurology
  • Arts and Humanities (miscellaneous)

Cite this

Huang, J., Han, C., Estacion, M., Vasylyev, D., Hoeijmakers, J. G. J., Gerrits, M. M., ... Waxman, S. G. (2014). Gain-of-function mutations in sodium channel NaV1.9 in painful neuropathy. Brain, 137(6), 1627-1642. https://doi.org/10.1093/brain/awu079

Gain-of-function mutations in sodium channel NaV1.9 in painful neuropathy. / Huang, Jianying; Han, Chongyang; Estacion, Mark; Vasylyev, Dymtro; Hoeijmakers, Janneke G J; Gerrits, Monique M.; Tyrrell, Lynda; Lauria, Giuseppe; Faber, Catharina G.; Dib-Hajj, Sulayman D.; Merkies, Ingemar S J; Waxman, Stephen G.

In: Brain, Vol. 137, No. 6, 2014, p. 1627-1642.

Research output: Contribution to journalArticle

Huang, J, Han, C, Estacion, M, Vasylyev, D, Hoeijmakers, JGJ, Gerrits, MM, Tyrrell, L, Lauria, G, Faber, CG, Dib-Hajj, SD, Merkies, ISJ & Waxman, SG 2014, 'Gain-of-function mutations in sodium channel NaV1.9 in painful neuropathy', Brain, vol. 137, no. 6, pp. 1627-1642. https://doi.org/10.1093/brain/awu079
Huang J, Han C, Estacion M, Vasylyev D, Hoeijmakers JGJ, Gerrits MM et al. Gain-of-function mutations in sodium channel NaV1.9 in painful neuropathy. Brain. 2014;137(6):1627-1642. https://doi.org/10.1093/brain/awu079
Huang, Jianying ; Han, Chongyang ; Estacion, Mark ; Vasylyev, Dymtro ; Hoeijmakers, Janneke G J ; Gerrits, Monique M. ; Tyrrell, Lynda ; Lauria, Giuseppe ; Faber, Catharina G. ; Dib-Hajj, Sulayman D. ; Merkies, Ingemar S J ; Waxman, Stephen G. / Gain-of-function mutations in sodium channel NaV1.9 in painful neuropathy. In: Brain. 2014 ; Vol. 137, No. 6. pp. 1627-1642.
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